Artery Research

Volume 4, Issue 4, December 2010, Pages 168 - 168

P7.03 DIFFERENT EFFECT OF NEURONAL AND ENDOTHELIAL NOS INHIBITION ON HEART, CORONARY AND CAROTID ARTERY OF WISTAR RATS

Authors
F. Kristek, S. Cacanyiova, M. Gerova
Institute of Normal and Pathological Physiology, Bratislava, Slovakia
Available Online 2 December 2010.
DOI
10.1016/j.artres.2010.10.080How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Long-term decrease of nitric oxide (NO) production evokes alterations in function and structure of cardiovascular system. We demonstrate the long-term effect of NO synthases inhibition with two different inhibitors on blood pressure (BP), heart and structure of coronary and carotid arteries. Four groups of ten weeks old Wistar rats were used: 1) control rats and rats receiving 2) NG-nitro-L-arginine methylester (L-NAME) (50 mg/kg/day in tap water), 3) 7-nitroindazole (7NI) (10 mg/kg/day in tap water), 4) the same doses of L-NAME (in tap water) and 7NI (in pellet diet). The experiment lasted 6 weeks. BP was measured by the plethysmographic method weekly. At the end of the experiment the animals were perfusion with a glutaraldehyde fixative (120 mmHg) and heart, coronary and carotid artery were excised and processed for electron microscopy.

Wistar L-NAME 7NI L-NAME+7NI
BP (mmHg) 112±2.20 164±2.10* 118±1,90+ 131±5,12*+#
H/B (mg/g) 3,08±0,14 3,41±0,13* 2,64±0,10*+ 3,07±0,12+#
Coronary a.
CSA (μm2) 8860±800 18280±1320* 6780±610*+ 14250±2290*+#
Carotid a.
CSA (μm2) 69190±2460 109780±4040* 54290±2200*+ 88950±4340*+#

Blood pressure (BP), heart/body weight (H/B), cross sectional area – intima+media (CSA).

*

P<0.01 vs. Wistar,

+

P<0.01 vs. L-NAME,

#

P<0.01 vs. 7NI

The study revealed, for the first time, that long-term inhibition of nNOS with 7NI, contrary to eNOS inhibition with nonspecific inhibitor L-NAME, evoked pressure independent cardiac hypotrophy and hypotrophy of the vessel wall. The data suggested that long-term nNOS inhibition likely iniciates a different chain of events leading to alterations in cardiovacular system then those after eNOS inhibition.

The study was supported by VEGA grant 2/0111/10, 2/0019/09.

Journal
Artery Research
Volume-Issue
4 - 4
Pages
168 - 168
Publication Date
2010/12/02
ISSN (Online)
1876-4401
ISSN (Print)
1872-9312
DOI
10.1016/j.artres.2010.10.080How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - JOUR
AU  - F. Kristek
AU  - S. Cacanyiova
AU  - M. Gerova
PY  - 2010
DA  - 2010/12/02
TI  - P7.03 DIFFERENT EFFECT OF NEURONAL AND ENDOTHELIAL NOS INHIBITION ON HEART, CORONARY AND CAROTID ARTERY OF WISTAR RATS
JO  - Artery Research
SP  - 168
EP  - 168
VL  - 4
IS  - 4
SN  - 1876-4401
UR  - https://doi.org/10.1016/j.artres.2010.10.080
DO  - 10.1016/j.artres.2010.10.080
ID  - Kristek2010
ER  -