Artery Research

Volume 4, Issue 4, December 2010, Pages 147 - 147

3.3 CARDIOTROPHIN-1 IS A DETERMINANT OF ARTERIAL STIFFNESS AND THICKNESS IN RODENTS

Authors
N. Lopez-Andres1, *, C. Laurent1, M.A. Fortuno2, C. Labat1, J. Diez2, F. Zannad1, 3, P. Lacolley1, P. Rossignol1, 3
1Inserm U961, Vandoeuvre-les-Nancy, France
2CIMA, Pamplona, Spain
3CIC Pierre Drouin, Vandoeuvre-les-Nancy, France
*Corresponding author.
Corresponding Author
N. Lopez-Andres
Available Online 2 December 2010.
DOI
10.1016/j.artres.2010.10.168How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Background & aims: Cardiotrophin-1 (CT-1), a cytokine belonging to the interleukin-6 family, exerts proliferative and secretory effects in vascular smooth muscle cells. We aimed to investigate the functional and morphological vascular changes induced by chronic CT-1 administration in rats and its involvement in the arterial phenotype of CT-1-null mice.

Methods: A) CT-1 (20μg/Kg, IP) was administrated to Wistar rats for six weeks (n=10/group). B) 2 year-old wild-type (WT) (n=12) and CT-1-null mice (n=12) were studied. Vascular structure and function were evaluated by an echo-tracking device. Circumferential wall stress, incremental elastic modulus (Einc), media cross-sectional area and pulse wave velocity (PWV) were measured. Aortic wall collagen, elastin and fibronectin contents were determined using immunohistochemistry, RT-PCR and Western blot.

Results: A) Neither vehicle nor CT-1 treatment modified BP. The Einc/wall stress curve from CT-1-treated was shifted to the left as compared with vehicle group. CT-1-treated rats also showed increased media cross sectional area (p<0.01), collagen content (p<0.01) and fibronectin expression (p<0.01). B) CT-1-null mice presented an increased wall stress (p<0.05) and Einc (p<0.05) as compared with WT mice. Media cross sectional area, collagen and fibronectin content were reduced (p<0.05) in mice lacking CT-1.

Conclusions: Normotensive rats subjected to CT-1 overloading developed impaired vascular function, characterized by an increment in arterial stiffness accompanied by an augmented media thickness and extracellular matrix production. Accordingly, CT-1-null mice presented a reduced arterial stiffness and a reduced media thickness and collagen content. Our data show that CT-1 is a key player in arterial thickness and stiffness.

Journal
Artery Research
Volume-Issue
4 - 4
Pages
147 - 147
Publication Date
2010/12/02
ISSN (Online)
1876-4401
ISSN (Print)
1872-9312
DOI
10.1016/j.artres.2010.10.168How to use a DOI?
Open Access
This is an open access article distributed under the CC BY-NC license.

Cite this article

TY  - JOUR
AU  - N. Lopez-Andres
AU  - C. Laurent
AU  - M.A. Fortuno
AU  - C. Labat
AU  - J. Diez
AU  - F. Zannad
AU  - P. Lacolley
AU  - P. Rossignol
PY  - 2010
DA  - 2010/12/02
TI  - 3.3 CARDIOTROPHIN-1 IS A DETERMINANT OF ARTERIAL STIFFNESS AND THICKNESS IN RODENTS
JO  - Artery Research
SP  - 147
EP  - 147
VL  - 4
IS  - 4
SN  - 1876-4401
UR  - https://doi.org/10.1016/j.artres.2010.10.168
DO  - 10.1016/j.artres.2010.10.168
ID  - Lopez-Andres2010
ER  -