3.3 CARDIOTROPHIN-1 IS A DETERMINANT OF ARTERIAL STIFFNESS AND THICKNESS IN RODENTS
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- 10.1016/j.artres.2010.10.168How to use a DOI?
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Background & aims: Cardiotrophin-1 (CT-1), a cytokine belonging to the interleukin-6 family, exerts proliferative and secretory effects in vascular smooth muscle cells. We aimed to investigate the functional and morphological vascular changes induced by chronic CT-1 administration in rats and its involvement in the arterial phenotype of CT-1-null mice.
Methods: A) CT-1 (20μg/Kg, IP) was administrated to Wistar rats for six weeks (n=10/group). B) 2 year-old wild-type (WT) (n=12) and CT-1-null mice (n=12) were studied. Vascular structure and function were evaluated by an echo-tracking device. Circumferential wall stress, incremental elastic modulus (Einc), media cross-sectional area and pulse wave velocity (PWV) were measured. Aortic wall collagen, elastin and fibronectin contents were determined using immunohistochemistry, RT-PCR and Western blot.
Results: A) Neither vehicle nor CT-1 treatment modified BP. The Einc/wall stress curve from CT-1-treated was shifted to the left as compared with vehicle group. CT-1-treated rats also showed increased media cross sectional area (p<0.01), collagen content (p<0.01) and fibronectin expression (p<0.01). B) CT-1-null mice presented an increased wall stress (p<0.05) and Einc (p<0.05) as compared with WT mice. Media cross sectional area, collagen and fibronectin content were reduced (p<0.05) in mice lacking CT-1.
Conclusions: Normotensive rats subjected to CT-1 overloading developed impaired vascular function, characterized by an increment in arterial stiffness accompanied by an augmented media thickness and extracellular matrix production. Accordingly, CT-1-null mice presented a reduced arterial stiffness and a reduced media thickness and collagen content. Our data show that CT-1 is a key player in arterial thickness and stiffness.
Cite this article
TY - JOUR AU - N. Lopez-Andres AU - C. Laurent AU - M.A. Fortuno AU - C. Labat AU - J. Diez AU - F. Zannad AU - P. Lacolley AU - P. Rossignol PY - 2010 DA - 2010/12/02 TI - 3.3 CARDIOTROPHIN-1 IS A DETERMINANT OF ARTERIAL STIFFNESS AND THICKNESS IN RODENTS JO - Artery Research SP - 147 EP - 147 VL - 4 IS - 4 SN - 1876-4401 UR - https://doi.org/10.1016/j.artres.2010.10.168 DO - 10.1016/j.artres.2010.10.168 ID - Lopez-Andres2010 ER -