P5.2 QUANTITATIVE PROTEOMICS REVEAL INCREASED CONTENT OF BASEMENT MEMBRANE PROTEINS IN ARTERIES FROM PATIENTS WITH TYPE 2 DIABETES, BUT REDUCED AMOUNTS AMONG METFORMIN USERS

S. Preil; L. Kristensen; H. Beck; P. Jensen; T. Steiniche; M. Bjørling-Poulsen; M. Larsen; M. Hansen; L. Rasmussen

doi:10.1016/j.artres.2014.09.139

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Volume 8, Issue 4, December 2014, Pages 143 - 143

P5.2 QUANTITATIVE PROTEOMICS REVEAL INCREASED CONTENT OF BASEMENT MEMBRANE PROTEINS IN ARTERIES FROM PATIENTS WITH TYPE 2 DIABETES, BUT REDUCED AMOUNTS AMONG METFORMIN USERS

Authors

S. Preil^a, L. Kristensen^a, H. Beck^a, P. Jensen^a, T. Steiniche^b, M. Bjørling-Poulsen^a, M. Larsen^c, M. Hansen^a, L. Rasmussen^a

^aOdense University Hospital, Odense, Denmark

^bUniversity Hospital of Aarhus, Aarhus, Denmark

^cUniversity of Southern Denmark, Odense, Denmark

Available Online 4 November 2014.

DOI: 10.1016/j.artres.2014.09.139 How to use a DOI?
Open Access: This is an open access article distributed under the CC BY-NC license.

We hypothesized that metformin intake influence the molecular composition of arterial tissue from patients with type 2 diabetes.

We analyzed non-atherosclerotic, internal mammary arteries, gathered at coronary by-pass operations from 30 patients with type 2 diabetes (16 treated with metformin, 14 without), as well as from 30 age- and gender-matched non-diabetic individuals. Quantitative proteome analysis was done by iTRAQ-labelling and LC-MS/MS analysis on individual trypsinized extracts of formalin fixed, paraffin embedded tissue. Sections were also analyzed by histology and immunohistochemistry.

We were able to quantitate 129 proteins. The amounts of the basement membrane (BM) component, alpha-1-type IV collagen were increased in patients with diabetes (0.96 +/− 0.05 (non-DM, n=30) vs 1.35 +/− 0.09 (T2DM, n=30), t-test: p= 0.00015, Benjamini-Hochberg correction: p=0.02), as was other BM-components, i.e. laminins and nidogen. The expression of type IV collagen, laminin and other altered proteins were significantly lower among metformin users, compared to patients not treated with metformin (alpha-1-type IV collagen: 1.63 +/− 0.1 (no metformin treatment, n=14)) vs 1.17 +/− 0.10 (metformin treated, n=16) (arb.units)), p=0.013). Patients treated with or without metformin had similar levels of HbA1c, cholesterol and blood pressure.

Accumulation of basement membrane proteins as part of the arteriopathy of type 2 diabetes link the diabetic macro- and micro-angiopathy and provides a molecular substrate for altered functions of the arteries in diabetes, as for example dysfunctional remodeling. Reduced amounts of basement membrane components in metformin treated individuals, despite similar glycemic control, are compatible with the hypothesis that metformin influence the vasculature.

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Journal: Artery Research
Volume-Issue: 8 - 4
Pages: 143 - 143
Publication Date: 2014/11/04
ISSN (Online): 1876-4401
ISSN (Print): 1872-9312
DOI: 10.1016/j.artres.2014.09.139 How to use a DOI?
Open Access: This is an open access article distributed under the CC BY-NC license.

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ris enw bib

TY  - JOUR
AU  - S. Preil
AU  - L. Kristensen
AU  - H. Beck
AU  - P. Jensen
AU  - T. Steiniche
AU  - M. Bjørling-Poulsen
AU  - M. Larsen
AU  - M. Hansen
AU  - L. Rasmussen
PY  - 2014
DA  - 2014/11/04
TI  - P5.2 QUANTITATIVE PROTEOMICS REVEAL INCREASED CONTENT OF BASEMENT MEMBRANE PROTEINS IN ARTERIES FROM PATIENTS WITH TYPE 2 DIABETES, BUT REDUCED AMOUNTS AMONG METFORMIN USERS
JO  - Artery Research
SP  - 143
EP  - 143
VL  - 8
IS  - 4
SN  - 1876-4401
UR  - https://doi.org/10.1016/j.artres.2014.09.139
DO  - 10.1016/j.artres.2014.09.139
ID  - Preil2014
ER  -

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