Nigella sativa Extract and Thymoquinone Regulate Inflammatory Cytokine and TET-2 Expression in Endothelial Cells

Jason Amartey; Samuel Gapper; Nurudeen Hussein; Keith Morris; Cathryn E. Withycombe

doi:10.2991/artres.k.191114.002

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Volume 25, Issue 3-4, December 2019, Pages 157 - 163

Nigella sativa Extract and Thymoquinone Regulate Inflammatory Cytokine and TET-2 Expression in Endothelial Cells

Authors

Jason Amartey¹, Samuel Gapper¹, Nurudeen Hussein¹^{, 2}, Keith Morris¹^{, *}, Cathryn E. Withycombe¹

¹Department of Biomedical Sciences, Cardiff Metropolitan University, Western Avenue, Cardiff, CF5 2YB, UK

²Technical Support, R&D, Ortho Clinical Diagnostics, Felindre Meadows, Pencoed, Bridgend, CF35 5PZ, UK

^*Corresponding author. Email: Kmorris@cardiffmet.ac.uk

Corresponding Author

Keith Morris

Received 1 November 2019, Accepted 11 November 2019, Available Online 2 December 2019.

DOI: 10.2991/artres.k.191114.002 How to use a DOI?
Keywords: Nigella sativa; TET-2; endothelial dysfunction; cytokine expression
Abstract: Numerous natural compounds including Nigella sativa (N. sativa) demonstrate anti-inflammatory and anti-diabetic antiangiogenic properties. Lipopolysaccharide (LPS) mediated inflammation is regarded as an important contributor to the inflammation that is associated with the development of arteriosclerosis. In this study, it was hypothesised that N. sativa Extract (NSE) and its main active component Thymoquinone (TQ) could potentially inhibit LPS mediated inflammatory cytokine secretion and monocyte recruitment factors and monocyte in Human Vascular Endothelial Cell (HECV) lines. In addition the Ten-Eleven Translocation (TET-2) an epigenetic regulator, increasingly regarded has having a major role in both the regulation of cytokine secretion and in the development of atherosclerosis through its ability to inhibit the inflammasome Nod-like Receptor Protein 3 (NLRP3) and Interleukin-1β (IL-1β) secretion was investigated. NSE significantly inhibited the production of both IL-6 and -8 and both NSE and TQ inhibited the gene expression of vascular endothelial growth factor and monocyte chemotactic protein-1 in HECV cells. NSE and TQ inhibited the gene expression of NLRP3 and IL-1β and significantly upregulated the gene expression of TET-2 in the presence of LPS. To conclude, NSE and TQ attenuated inflammatory and monocyte recruitment response and also demonstrate a potentially important role in regulating both NLRP3 and TET-2 expression.
Copyright: © 2019 Association for Research into Arterial Structure and Physiology. Publishing services by Atlantis Press International B.V.
Open Access: This is an open access article distributed under the CC BY-NC 4.0 license (http://creativecommons.org/licenses/by-nc/4.0/).

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Journal: Artery Research
Volume-Issue: 25 - 3-4
Pages: 157 - 163
Publication Date: 2019/12/02
ISSN (Online): 1876-4401
ISSN (Print): 1872-9312
DOI: 10.2991/artres.k.191114.002 How to use a DOI?
Open Access: This is an open access article distributed under the CC BY-NC 4.0 license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

ris enw bib

TY  - JOUR
AU  - Jason Amartey
AU  - Samuel Gapper
AU  - Nurudeen Hussein
AU  - Keith Morris
AU  - Cathryn E. Withycombe
PY  - 2019
DA  - 2019/12/02
TI  - Nigella sativa Extract and Thymoquinone Regulate Inflammatory Cytokine and TET-2 Expression in Endothelial Cells
JO  - Artery Research
SP  - 157
EP  - 163
VL  - 25
IS  - 3-4
SN  - 1876-4401
UR  - https://doi.org/10.2991/artres.k.191114.002
DO  - 10.2991/artres.k.191114.002
ID  - Amartey2019
ER  -

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