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Volume 1, Issue 2, April 2020, Pages 140 - 146

In vivo Modulatory Effect of Coffee (Coffea canephora var. Robusta) on the Expression Levels of Murine microRNA-124-3p Associated with Antioxidant Defenses

Authors
Valeria Curti1, Manuela Verri2, Alessandra Baldi1, Marco Dacrema1, Irene Masiello1, Maurizia Dossena2, Maria Daglia3, 4, *
1Department of Drug Sciences, Medicinal Chemistry and Pharmaceutical Technology Section, The University of Pavia, Pavia, Italy
2Department of Biology and Biotechnology “Lazzaro Spallanzani”, The University of Pavia, Pavia, Italy
3Department of Pharmacy, University of Naples Federico II, 80131 Naples, Italy
4International Research Center for Food Nutrition and Safety, Jiangsu University, Zhenjiang 212013, China
*Corresponding author. Email: maria.daglia@unina.it
Corresponding Author
Maria Daglia
Received 30 May 2019, Accepted 28 June 2019, Available Online 23 August 2019.
DOI
10.2991/efood.k.190802.001How to use a DOI?
Keywords
Epigenetics; Coffea canephora var. Robusta; in vivo study
Abstract

Antioxidant activity is one of the most studied properties of coffee as a beverage, and is mainly ascribed to its polyphenol content, although there is limited scientific literature regarding its mechanism of action at the molecular level. In recent years, epigenetics has been featured as a promising approach in many studies for explaining the mechanisms of action involved in its effects on health exerted by foods and food components. To investigate such an epigenetic contribution toward the antioxidant properties of coffee, an in vivo study was performed on healthy C57BL/6 mice fed with a normal diet supplemented with a low-molecular-weight (MW) fraction (MW < 3500 Da) obtained from regular and decaffeinated coffee. Regular coffee supplementation induced a downregulation of microRNA-124-3p, and its validated target, mRNA coding for glutathione peroxidase (GPX), was upregulated. Decaffeinated coffee was not found to induce any significant changes in the expression levels of microRNA-124-3p and its validated target mRNA. It may be concluded from the results that regular intake of coffee exerts its antioxidant activity through an epigenetic mechanism of action, at least in part, being able to influence the expression levels on a miRNA and an mRNA involved in antioxidant defenses, with caffeine playing a crucial role.

Copyright
© 2019 International Association of Dietetic Nutrition and Safety. Publishing services by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license (http://creativecommons.org/licenses/by-nc/4.0/).

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<Previous Article In Issue
Next Article In Issue>
Journal
eFood
Volume-Issue
1 - 2
Pages
140 - 146
Publication Date
2019/08/23
ISSN (Online)
2666-3066
DOI
10.2991/efood.k.190802.001How to use a DOI?
Copyright
© 2019 International Association of Dietetic Nutrition and Safety. Publishing services by Atlantis Press International B.V.
Open Access
This is an open access article distributed under the CC BY-NC 4.0 license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

risenwbib
TY  - JOUR
AU  - Valeria Curti
AU  - Manuela Verri
AU  - Alessandra Baldi
AU  - Marco Dacrema
AU  - Irene Masiello
AU  - Maurizia Dossena
AU  - Maria Daglia
PY  - 2019
DA  - 2019/08/23
TI  - In vivo Modulatory Effect of Coffee (Coffea canephora var. Robusta) on the Expression Levels of Murine microRNA-124-3p Associated with Antioxidant Defenses
JO  - eFood
SP  - 140
EP  - 146
VL  - 1
IS  - 2
SN  - 2666-3066
UR  - https://doi.org/10.2991/efood.k.190802.001
DO  - 10.2991/efood.k.190802.001
ID  - Curti2019
ER  -