Proceedings of the International Scientific Conference The Fifth Technological Order: Prospects for the Development and Modernization of the Russian Agro-Industrial Sector (TFTS 2019)

Symbiotic Efficacy of Variety-Specific Strains of Nitrogen-Fixing Bacteria on Soybean Varieties of Omsk Selection

Authors
Lyudmila Omelyanyuk, Akimbek Asanov, Alyona Karmazina, Oksana Yusova
Corresponding Author
Lyudmila Omelyanyuk
Available Online 29 January 2020.
DOI
10.2991/assehr.k.200113.181How to use a DOI?
Keywords
variety, biological product, nitrogen-fixing nodules, plant habit, seed yield, protein and fat content
Abstract

The experiments were performed in 2017-2018 in the fields of the Omsk Agrarian Scientific Center (Omsk, Russian Federation), with winter cereals as fore corp. The objective was to study and isolate strains of varietal-specific nodule bacteria that positively affect the development of soybean plants and nitrogen-fixing nodules. The experiment included soybean varieties of northern ecotype of the Omsk ASC selection: Zolotistaya and Cheremshanka. The seeds of cultivars were processed before sowing with microbiological fertilizers – four experimental varietal-specific isolates of nodule bacteria created in the Laboratory of Industrial Microbiology “PlantaPlus” (Tomsk, Russian Federation); control (K) experiment – clear water. Structural analysis (10 plants for each option) was carried out in late flowering (July) and in the phase of bean filling (August). Harvesting was in the 3rd decade of September with the Hege 125 combine. Biochemical parameters of seeds were determined in the Laboratory of pPlant Genetics, Physiology and Biochemistry at the Omsk ASC: nitrogen content – by automatic analyzer “KjeltekAuto 1030 Analyzer”, nitrogen to protein conversion factor for soybean grain – 6,25; the content of crude fat is by the Soxhlet extractor. It was established that soybean varieties differ in the strength of response to the use of biological products, which are mainly effective. The Cheremshanka variety has the highest weight of plants and nitrogen-fixing nodules and seed yield in comparison with the Zolotistaya variety. The maximum experimental values (on average over 2 years) without loss of seed quality were obtained in the Cheremshanka cultivar variant The experiments were performed in 2017-2018 in the fields of the Omsk Agrarian Scientific Center (Omsk, Russian Federation), with winter cereals as fore corp. The objective was to study and isolate strains of varietal-specific nodule bacteria that positively affect the development of soybean plants and nitrogen-fixing nodules. The experiment included soybean varieties of northern ecotype of the Omsk ASC selection: Zolotistaya and Cheremshanka. The seeds of cultivars were processed before sowing with microbiological fertilizers – four experimental varietal-specific isolates of nodule bacteria created in the Laboratory of Industrial Microbiology “PlantaPlus” (Tomsk, Russian Federation); control (K) experiment – clear water. Structural analysis (10 plants for each option) was carried out in late flowering (July) and in the phase of bean filling (August). Harvesting was in the 3rd decade of September with the Hege 125 combine. Biochemical parameters of seeds were determined in the Laboratory of pPlant Genetics, Physiology and Biochemistry at the Omsk ASC: nitrogen content – by automatic analyzer “KjeltekAuto 1030 Analyzer”, nitrogen to protein conversion factor for soybean grain – 6,25; the content of crude fat is by the Soxhlet extractor. It was established that soybean varieties differ in the strength of response to the use of biological products, which are mainly effective. The Cheremshanka variety has the highest weight of plants and nitrogen-fixing nodules and seed yield in comparison with the Zolotistaya variety. The maximum experimental values (on average over 2 years) without loss of seed quality were obtained in the Cheremshanka cultivar variant + strain C.11/2017: the green mass of the plant at the end of flowering is 43,9 g (The experiments were performed in 2017-2018 in the fields of the Omsk Agrarian Scientific Center (Omsk, Russian Federation), with winter cereals as fore corp. The objective was to study and isolate strains of varietal-specific nodule bacteria that positively affect the development of soybean plants and nitrogen-fixing nodules. The experiment included soybean varieties of northern ecotype of the Omsk ASC selection: Zolotistaya and Cheremshanka. The seeds of cultivars were processed before sowing with microbiological fertilizers – four experimental varietal-specific isolates of nodule bacteria created in the Laboratory of Industrial Microbiology “PlantaPlus” (Tomsk, Russian Federation); control (K) experiment – clear water. Structural analysis (10 plants for each option) was carried out in late flowering (July) and in the phase of bean filling (August). Harvesting was in the 3rd decade of September with the Hege 125 combine. Biochemical parameters of seeds were determined in the Laboratory of pPlant Genetics, Physiology and Biochemistry at the Omsk ASC: nitrogen content – by automatic analyzer “KjeltekAuto 1030 Analyzer”, nitrogen to protein conversion factor for soybean grain – 6,25; the content of crude fat is by the Soxhlet extractor. It was established that soybean varieties differ in the strength of response to the use of biological products, which are mainly effective. The Cheremshanka variety has the highest weight of plants and nitrogen-fixing nodules and seed yield in comparison with the Zolotistaya variety. The maximum experimental values (on average over 2 years) without loss of seed quality were obtained in the Cheremshanka cultivar variant + strain C.11/2017: the green mass of the plant at the end of flowering is 43,9 g (+13,6 g to K); at the bean filling phase – 90,1 g (The experiments were performed in 2017-2018 in the fields of the Omsk Agrarian Scientific Center (Omsk, Russian Federation), with winter cereals as fore corp. The objective was to study and isolate strains of varietal-specific nodule bacteria that positively affect the development of soybean plants and nitrogen-fixing nodules. The experiment included soybean varieties of northern ecotype of the Omsk ASC selection: Zolotistaya and Cheremshanka. The seeds of cultivars were processed before sowing with microbiological fertilizers – four experimental varietal-specific isolates of nodule bacteria created in the Laboratory of Industrial Microbiology “PlantaPlus” (Tomsk, Russian Federation); control (K) experiment – clear water. Structural analysis (10 plants for each option) was carried out in late flowering (July) and in the phase of bean filling (August). Harvesting was in the 3rd decade of September with the Hege 125 combine. Biochemical parameters of seeds were determined in the Laboratory of pPlant Genetics, Physiology and Biochemistry at the Omsk ASC: nitrogen content – by automatic analyzer “KjeltekAuto 1030 Analyzer”, nitrogen to protein conversion factor for soybean grain – 6,25; the content of crude fat is by the Soxhlet extractor. It was established that soybean varieties differ in the strength of response to the use of biological products, which are mainly effective. The Cheremshanka variety has the highest weight of plants and nitrogen-fixing nodules and seed yield in comparison with the Zolotistaya variety. The maximum experimental values (on average over 2 years) without loss of seed quality were obtained in the Cheremshanka cultivar variant + strain C.11/2017: the green mass of the plant at the end of flowering is 43,9 g (+13,6 g to K); at the bean filling phase – 90,1 g (+32,9 g to K); seed yield is 3,56 t/ha (The experiments were performed in 2017-2018 in the fields of the Omsk Agrarian Scientific Center (Omsk, Russian Federation), with winter cereals as fore corp. The objective was to study and isolate strains of varietal-specific nodule bacteria that positively affect the development of soybean plants and nitrogen-fixing nodules. The experiment included soybean varieties of northern ecotype of the Omsk ASC selection: Zolotistaya and Cheremshanka. The seeds of cultivars were processed before sowing with microbiological fertilizers – four experimental varietal-specific isolates of nodule bacteria created in the Laboratory of Industrial Microbiology “PlantaPlus” (Tomsk, Russian Federation); control (K) experiment – clear water. Structural analysis (10 plants for each option) was carried out in late flowering (July) and in the phase of bean filling (August). Harvesting was in the 3rd decade of September with the Hege 125 combine. Biochemical parameters of seeds were determined in the Laboratory of pPlant Genetics, Physiology and Biochemistry at the Omsk ASC: nitrogen content – by automatic analyzer “KjeltekAuto 1030 Analyzer”, nitrogen to protein conversion factor for soybean grain – 6,25; the content of crude fat is by the Soxhlet extractor. It was established that soybean varieties differ in the strength of response to the use of biological products, which are mainly effective. The Cheremshanka variety has the highest weight of plants and nitrogen-fixing nodules and seed yield in comparison with the Zolotistaya variety. The maximum experimental values (on average over 2 years) without loss of seed quality were obtained in the Cheremshanka cultivar variant + strain C.11/2017: the green mass of the plant at the end of flowering is 43,9 g (+13,6 g to K); at the bean filling phase – 90,1 g (+32,9 g to K); seed yield is 3,56 t/ha (+0,19 t/ha to K), weight of nitrogen-fixing nodules 1,27 g/plant (The experiments were performed in 2017-2018 in the fields of the Omsk Agrarian Scientific Center (Omsk, Russian Federation), with winter cereals as fore corp. The objective was to study and isolate strains of varietal-specific nodule bacteria that positively affect the development of soybean plants and nitrogen-fixing nodules. The experiment included soybean varieties of northern ecotype of the Omsk ASC selection: Zolotistaya and Cheremshanka. The seeds of cultivars were processed before sowing with microbiological fertilizers – four experimental varietal-specific isolates of nodule bacteria created in the Laboratory of Industrial Microbiology “PlantaPlus” (Tomsk, Russian Federation); control (K) experiment – clear water. Structural analysis (10 plants for each option) was carried out in late flowering (July) and in the phase of bean filling (August). Harvesting was in the 3rd decade of September with the Hege 125 combine. Biochemical parameters of seeds were determined in the Laboratory of pPlant Genetics, Physiology and Biochemistry at the Omsk ASC: nitrogen content – by automatic analyzer “KjeltekAuto 1030 Analyzer”, nitrogen to protein conversion factor for soybean grain – 6,25; the content of crude fat is by the Soxhlet extractor. It was established that soybean varieties differ in the strength of response to the use of biological products, which are mainly effective. The Cheremshanka variety has the highest weight of plants and nitrogen-fixing nodules and seed yield in comparison with the Zolotistaya variety. The maximum experimental values (on average over 2 years) without loss of seed quality were obtained in the Cheremshanka cultivar variant + strain C.11/2017: the green mass of the plant at the end of flowering is 43,9 g (+13,6 g to K); at the bean filling phase – 90,1 g (+32,9 g to K); seed yield is 3,56 t/ha (+0,19 t/ha to K), weight of nitrogen-fixing nodules 1,27 g/plant (+1,00 g to K).

Copyright
© 2020, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the International Scientific Conference The Fifth Technological Order: Prospects for the Development and Modernization of the Russian Agro-Industrial Sector (TFTS 2019)
Series
Advances in Social Science, Education and Humanities Research
Publication Date
29 January 2020
ISBN
978-94-6252-886-4
ISSN
2352-5398
DOI
10.2991/assehr.k.200113.181How to use a DOI?
Copyright
© 2020, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Lyudmila Omelyanyuk
AU  - Akimbek Asanov
AU  - Alyona Karmazina
AU  - Oksana Yusova
PY  - 2020
DA  - 2020/01/29
TI  - Symbiotic Efficacy of Variety-Specific Strains of Nitrogen-Fixing Bacteria on Soybean Varieties of Omsk Selection
BT  - Proceedings of the International Scientific Conference The Fifth Technological Order: Prospects for the Development and Modernization of the Russian Agro-Industrial Sector (TFTS 2019)
PB  - Atlantis Press
SP  - 258
EP  - 262
SN  - 2352-5398
UR  - https://doi.org/10.2991/assehr.k.200113.181
DO  - 10.2991/assehr.k.200113.181
ID  - Omelyanyuk2020
ER  -