Proceedings of the 4th Science and Mathematics International Conference (SMIC 2024)

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Assessing the ecpA Gene as a Target for Detecting Klebsiella pneumoniae Using Real-time Polymerase Chain Reaction Method

Authors
Muktiningsih Nurjayadi1, 2, *, Agus Setiawan1, 2, Ananda Indah Putri Sihombing1, 2, Anisa Fitriyanti1, 2, Grace Grace1, 2, Gusti Angieta Putri1, 2, Helzi Angelina1, 2, Puan Aqila Azizah1, 2, Royna Rahma Musie1, 2, Jefferson Lynford Declan1, 2, Gladys Indira Putri1, 2, Dandy Akbar Juliansyah1, 2, Siti Fatimah2, Atikah Nur Rahmawati1, 2, Irma Ratna Kartika1, 2, Fera Kurniadewi1, 2, Irwan Saputra1, 2, Bassam Abomoelak3, Hesham A. El Enshasy4, 5, 6
1Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Negeri Jakarta, Gedung KH. Hasjim Asj’ari, 6Th Floor, Jl. Rawamangun Muka, Jakarta Timur, 13220, Indonesia
2Research Center for Detection of Pathogenic Bacteria, Lembaga Penelitian Dan Pengabdian Kepada Masyarakat, Universitas Negeri Jakarta, Jl. Rawamangun Muka, Jakarta Timur, 13220, Indonesia
3Arnold Palmer Hospital Pediatric Specialty Diagnostic Laboratory, Orlando, FL, 32806, USA
4Innovation Center in Agritechnology for Advanced Bioprocessing (ICA), Universiti Teknologi Malaysia (UTM), Pagoh, Johor, Malaysia
5School of Chemical and Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia (UTM), Skudai, Johor Bahru, Malaysia
6City of Scientific Research and Technology Applications, New Burg Al Arab, Alexandria, Egypt
*Corresponding author. Email: muktiningsih@unj.ac.id
Corresponding Author
Muktiningsih Nurjayadi
Available Online 26 December 2024.
DOI
10.2991/978-94-6463-624-6_11How to use a DOI?
Keywords
Klebsiella pneumoniae; Pathogenic Bacteria; ecpA Gene; real-time Polymerase Chain Reaction
Abstract

Klebsiella pneumoniae is the most nosocomial pathogenic bacteria found in the hospital that causes infections such as pneumonia, liver abscesses, and urinary tract infections. The ecpA gene is one of the target genes of Klebsiella pneumoniae that functions in biofilm development to increase the ability of bacteria to colonize and as the main subunit of fimbriae. This study aims to determine the confirmation, sensitivity, and specificity test of pathogenic bacteria by real-time Polymerase Chain Reaction method. The ecpA primer has an amplicons length of 170 bp and has an annealing temperature of 60℃. These primer pair produced Ct test at 16.78±0.04 and Tm value of 86.33℃±0.12. The specificity test of the ecpA gene successfully differentiated between target and non-target bacteria based on Ct and Tm value. Therefore, these primers can detect Klebsiella pneumoniae bacteria at the smallest concentration until 1.06 pg/µL equivalent to 1.47 x 104 CFU. Based on these results, the ecpA primer successfully detected Klebsiella pneumoniae bacteria quickly, specifically and sensitively using the rt-PCR method. In the next step, these primer pairs can detect the artificial contaminated samples using rt-PCR method.

Copyright
© 2024 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the 4th Science and Mathematics International Conference (SMIC 2024)
Series
Advances in Physics Research
Publication Date
26 December 2024
ISBN
978-94-6463-624-6
ISSN
2352-541X
DOI
10.2991/978-94-6463-624-6_11How to use a DOI?
Copyright
© 2024 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

risenwbib
TY  - CONF
AU  - Muktiningsih Nurjayadi
AU  - Agus Setiawan
AU  - Ananda Indah Putri Sihombing
AU  - Anisa Fitriyanti
AU  - Grace Grace
AU  - Gusti Angieta Putri
AU  - Helzi Angelina
AU  - Puan Aqila Azizah
AU  - Royna Rahma Musie
AU  - Jefferson Lynford Declan
AU  - Gladys Indira Putri
AU  - Dandy Akbar Juliansyah
AU  - Siti Fatimah
AU  - Atikah Nur Rahmawati
AU  - Irma Ratna Kartika
AU  - Fera Kurniadewi
AU  - Irwan Saputra
AU  - Bassam Abomoelak
AU  - Hesham A. El Enshasy
PY  - 2024
DA  - 2024/12/26
TI  - Assessing the ecpA Gene as a Target for Detecting Klebsiella pneumoniae Using Real-time Polymerase Chain Reaction Method
BT  - Proceedings of the 4th Science and Mathematics International Conference (SMIC 2024)
PB  - Atlantis Press
SP  - 112
EP  - 128
SN  - 2352-541X
UR  - https://doi.org/10.2991/978-94-6463-624-6_11
DO  - 10.2991/978-94-6463-624-6_11
ID  - Nurjayadi2024
ER  -