Development of Rat Model with Iron Deficiency Anemia by Modification of Its Standard Food

DOI

10.2991/phico-17.2018.22How to use a DOI?

Keywords

Rats model; iron deficiency anemia; hemoglobin levels

Abstract

Anemia is a nutritional problem in the world which is mainly caused iron deficiency and its global prevalence reaches 29-43%. Animal models with iron deficiency anemia (IDA) have been generated in some research centers but they used different standard diets and need longer time. Therefore the aim of this study was to investigate reduction of hemoglobin (Hb) levels in rat model with IDA. This study used 24 female Wistar rats which aged 2 months old and had ± 200g body weight and Hb levels >13 g/dl in the Laboratory of Food and Nutrition Study Center, Gadjah Mada University for 22 days. Each group consisted of 6 rats and control group (C) got AIN 93M standard food for 15 days. While treatment groups (T) got low iron diet of AIN 93M food for 5 (T1), 10 (T2) and 15 (T3) days. Hb levels were measured using the cyanmethemoglobin method. All collected data were analyzed using independent and paired t-student, Anova followed by Tukey post hoc and Mann Whitney tests with p value < 0.05. T groups significantly had lower Hb levels than the control group (p<0.001). The lowest Hb levels (8,5 ± 0,4g/dl) were observed in T3 group. The mean different of Hb levels in T3 group (5,7 ± 0,2g/dl) was significantly higher than in T1 and T2 groups. Administration of low iron diet of food standard decreases rat Hb levels less than 10g/dl in 10 or 15 days.

Copyright

© 2018, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Tri Susanti
AU  - Paramasari Dirgahayu
AU  - Dono Indarto
PY  - 2017/12
DA  - 2017/12
TI  - Development of Rat Model with Iron Deficiency Anemia by Modification of Its Standard Food
BT  - Proceedings of the 2nd Public Health International Conference (PHICo 2017)
PB  - Atlantis Press
SP  - 107
EP  - 110
SN  - 2468-5739
UR  - https://doi.org/10.2991/phico-17.2018.22
DO  - 10.2991/phico-17.2018.22
ID  - Susanti2017/12
ER  -