Proceedings of the 2015 International Conference on Power Electronics and Energy Engineering

Gefitinib, as a new stent coating material, specifically inhibits smooth muscle cells proliferation through inhibition of EGFR/Akt pathway phosphorylation

Authors
F. Li, S.Y. Wang, J. Luo, Z.X. Wu, T. Xiao, O. Zeng, J. Yang, C. Chu
Corresponding Author
F. Li
Available Online April 2015.
DOI
10.2991/peee-15.2015.60How to use a DOI?
Keywords
Coating material, Cytotoxicity, Stent, Gefitinib, Smooth muscle cells, Phosphorylation.
Abstract

The objective of this paper is to investigate the effects of gefitinib as a new stent coating material on proliferation of smooth muscle cells and the expression and phosphorylation of EGFR/Akt protein. Rat smooth muscle cells were cultured in medium with gefitinib (10-2 mol/L-10 mol/L) for 24h-72h. MTT assay was used to test the inhibition of cell proliferation. Western-blot was used to detect the expression of EGFR, Akt, phosphorylated EGFR (p-EGFR) and phosphorylated Akt (p-Akt). MTT assay showed that the inhibitory effect of gefitinib on smooth muscle cells’ proliferation was in a time and concentration dependent manner. Western-blot showed the expression of EGFR and Akt has no significant change between gefitinib group and paclitaxel group in smooth muscle cells, but gefitinib could significantly inhibit the phosphorylation of EGFR and Akt in smooth muscle cells compared with paclitaxel. It is concluded that Gefitinib could significantly suppress the proliferation of smooth muscle cells; the mechanism might be by inhibiting the phosphorylation of EGFR and Akt.

Copyright
© 2015, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 2015 International Conference on Power Electronics and Energy Engineering
Series
Advances in Engineering Research
Publication Date
April 2015
ISBN
978-94-62520-83-7
ISSN
2352-5401
DOI
10.2991/peee-15.2015.60How to use a DOI?
Copyright
© 2015, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - F. Li
AU  - S.Y. Wang
AU  - J. Luo
AU  - Z.X. Wu
AU  - T. Xiao
AU  - O. Zeng
AU  - J. Yang
AU  - C. Chu
PY  - 2015/04
DA  - 2015/04
TI  - Gefitinib, as a new stent coating material, specifically inhibits smooth muscle cells proliferation through inhibition of EGFR/Akt pathway phosphorylation
BT  - Proceedings of the 2015 International Conference on Power Electronics and Energy Engineering
PB  - Atlantis Press
SP  - 225
EP  - 227
SN  - 2352-5401
UR  - https://doi.org/10.2991/peee-15.2015.60
DO  - 10.2991/peee-15.2015.60
ID  - Li2015/04
ER  -