Genetic Diversity of Mycobacterium tuberculosis complex isolates circulating in North Central Nigeria

DOI

10.2991/978-94-6463-306-1_5How to use a DOI?

Keywords

Nigeria; Mycobacterium tuberculosis; MIRU-VNTR; high diversity

Abstract

With the higher risk of tuberculosis associated with developing countries, it has increasingly become imperative to determine the genetic diversity of strains of the Mycobacterium tuberculosis complex (MTBC). This is essential for establishing control programs as it provides information on possible sources and chains of transmission.

This study aimed to assess the genetic diversity of M. tuberculosis complex strains isolated from the North Central Nigeria using the 12-locus MIRU-VNTR.

Mycobacterium tuberculosis complex isolates (106) obtained from a previous study between November 2017 and December 2018 were characterized using the 12-locus mycobacterial interspersed repetitive unit variable number tandem repeats (MIRU-VNTR) typing method. Data generated were then analyzed using the MIRU-VNTRplus web application to determine the allelic diversity.

Allelic diversity as determined by the Hunter-Gaston diversity index showed a variation from 0.21 to 0.81 associated with MIRU locus 2 and 26 respectively. In total, 41.7% of the loci were highly discriminant, 41.7% moderately discriminant, and 16.7% were poorly discriminant. MIRU-VNTR typing revealed a high diversity, with a clustering rate of 1.9% and a total of ten different genotypes. UgandaII (Lineage 4) was the most common (14, 21.2%), followed by Bovis (10, 15.1%) and Cameroon (6, 9.1%); UgandaI (Lineage 4) was the least common (1, 1.5%).

The 12-locus MIRU-VNTR provided an adequate typing tool for assessing the genetic diversity of MTBC in this study as evidenced by the 0.94 HGDI value for total isolates. We report a high diversity of MTBC strains circulating in the North Central region of Nigeria, indicating multiple introductions of this pathogen to this region and a lack of clonal spread.

Copyright

© 2023 The Author(s)

Open Access

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TY  - CONF
AU  - Stella S. Makpu
AU  - Kome Otokunefor
AU  - Tosanwumi Vincent Otokunefor
PY  - 2023
DA  - 2023/12/18
TI  - Genetic Diversity of Mycobacterium tuberculosis complex isolates circulating in North Central Nigeria
BT  - Proceedings of the 6th Biennial Conference of Organization for Women in Science for the Developing World Nigeria (OWSD 2023)
PB  - Atlantis Press
SP  - 69
EP  - 86
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-306-1_5
DO  - 10.2991/978-94-6463-306-1_5
ID  - Makpu2023
ER  -