Antibiotic Sensitivity Pattern of Escherichia coli Isolates from Urine and Stool of Apparently Healthy Individuals in Port Harcourt Nigeria

DOI

10.2991/978-94-6463-306-1_23How to use a DOI?

Keywords

Escherichia coli; antibiotics; resistance; MAR index and MDR

Abstract

Antibiotic-resistant strains of once-controllable pathogens are emerging and spreading beyond the healthcare system. It places an immense economic strain on the healthcare system and raises serious concerns about infection management and prevention. This research aims to identify any trends in antibiotic resistance among Escherichia coli (E. coli) strains recovered from urine and stool of outpatients of the University of Port Harcourt Teaching Hospital. E. coli was isolated and identified using standard laboratory techniques. Antimicrobial susceptibility testing was performed using the modified Kirby - Bauer disc diffusion method, and results were reported using an interpretive chart developed by the Clinical and Laboratory Standards Institute (CLSI). The proportion of antibiotics to which an isolated strain is resistant was used to establish the Multiple Antibiotic Resistance Index (MAR). A total of 62 E. coli isolates were identified, with a large proportion (>70%) resistant to cefuroxime, cefotaxime, cefixime, ceftriaxone, imipenem, ampiclox, and amoxicilin clavulanate, intermediately susceptible to nalidixic acid and gentamycin, and very sensitive to nitrofurantoin, ofloxacin, and levofloxacin in both urine and stool. All isolates were multi-drug resistant (MDR) to at least 3 classes of antibiotics. Hundred percent of the samples with a MAR index > 0.2. A high MAR index in E. coli isolates suggests that these bacteria have been exposed to antibiotics in the past and have evolved resistance to routinely used antibiotics; thus, antimicrobial susceptibility testing is crucial in deciding on a course of treatment. Spread among people can be slowed by focusing on proper infection prevention and control measures.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - S. Adewuyi-Oseni
AU  - E. O. Agbagwa
AU  - K. Otokunefor
AU  - N. Frank-Peterside
PY  - 2023
DA  - 2023/12/18
TI  - Antibiotic Sensitivity Pattern of Escherichia coli Isolates from Urine and Stool of Apparently Healthy Individuals in Port Harcourt Nigeria
BT  - Proceedings of the 6th Biennial Conference of Organization for Women in Science for the Developing World Nigeria (OWSD 2023)
PB  - Atlantis Press
SP  - 343
EP  - 356
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-306-1_23
DO  - 10.2991/978-94-6463-306-1_23
ID  - Adewuyi-Oseni2023
ER  -