Proceedings of the 2nd Global Health and Innovation in conjunction with 6th ORL Head and Neck Oncology Conference (ORLHN 2021)

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Cell Multiplication in Platelet Rich Fibrin (PRF) and Platelet Rich Plasma (PRP) as Biofuels for Cartilage Tissue Engineering

Authors
Ahmad Taufik S1, 2, *, Adnanto Wiweko1, 2, Devi Rahmadona1, 2, Dyah Purnaning1, Mohammad Rizki1, 2, Bayu Tirta Dirja1, 2
1Faculty of Medicine, University of Mataram, Indonesia
2Stem Cell and Regenerative Medicine Center, Mataram University Teaching Hospital, Indonesia
*Corresponding author. Email: taufik.unram@gmail.com
Corresponding Author
Ahmad Taufik S
Available Online 21 February 2022.
DOI
10.2991/ahsr.k.220206.062How to use a DOI?
Keywords
Cartilage engineering; Platelet rich fibrin; platelet rich plasma; Growth factor
Abstract

Cartilage injury remains a major orthopedic challenge due to its low repair capacity, with the tendency to cause osteoarthritis. In addition, the treatment method involving platelet rich plasma (PRP) and platelet rich fibrin (PRF) as cartilage engineering components greatly anticipates promising recovery results. This research is aimed at determining the number of cells in platelet rich plasma (PRP) and platelet rich fibrin (PRF), particularly inflammatory cells. Blood specimens were collected from 30 rabbits that were divided into three treatment groups. The samples were then processed into control, PRP and PRF. This was followed by determining the number of cells in the form of platelets, leukocytes, lymphocytes, neutrophils and monocytes. According to the blood test results, the number of platelets increased by 3.8 and 4.5-fold in the PRP and PRF groups, respectively. Furthermore, 2 times (2.1-fold) increment was observed in leukocyte concentration for PRF, but zero in PRP. Conversely, the number of lymphocytes, neutrophils and monocytes decreased to 82, 72 and 40%, correspondingly, while PRP still showed no change. Based on the overall results and discussion, the number of platelets in both PRP and PRF increased severally, while leukocyte only occurred in PRF. This multi-fold expansion accompanied by growth factors has the capacity to accelerate cartilage recovery. Furthermore, the presence of inflammatory cells enhances the healing potential already existing in PRF.

Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article under the CC BY-NC license.

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Volume Title
Proceedings of the 2nd Global Health and Innovation in conjunction with 6th ORL Head and Neck Oncology Conference (ORLHN 2021)
Series
Advances in Health Sciences Research
Publication Date
21 February 2022
ISBN
978-94-6239-540-4
ISSN
2468-5739
DOI
10.2991/ahsr.k.220206.062How to use a DOI?
Copyright
© 2022 The Authors. Published by Atlantis Press International B.V.
Open Access
This is an open access article under the CC BY-NC license.

Cite this article

risenwbib
TY  - CONF
AU  - Ahmad Taufik S
AU  - Adnanto Wiweko
AU  - Devi Rahmadona
AU  - Dyah Purnaning
AU  - Mohammad Rizki
AU  - Bayu Tirta Dirja
PY  - 2022
DA  - 2022/02/21
TI  - Cell Multiplication in Platelet Rich Fibrin (PRF) and Platelet Rich Plasma (PRP) as Biofuels for Cartilage Tissue Engineering
BT  - Proceedings of the 2nd Global Health and Innovation in conjunction with 6th ORL Head and Neck Oncology Conference (ORLHN 2021)
PB  - Atlantis Press
SP  - 331
EP  - 335
SN  - 2468-5739
UR  - https://doi.org/10.2991/ahsr.k.220206.062
DO  - 10.2991/ahsr.k.220206.062
ID  - TaufikS2022
ER  -