Bioinformatics Analysis of the Phytoene Dehydrogenase Gene in Cabbage (Brassica Oleracea Var. Capitata)

DOI

10.2991/mseee-18.2018.54How to use a DOI?

Keywords

Phytoene dehydrogenase; bioinformatics analysis; Subcellular localization.

Abstract

Phytoene dehydrogenase (PDS) is an important enzyme in carotenoid biosynthesis. Here, the Brassica oleracea var. capitata PDS (BocPDS) gene sequences were obtained from Brassica database (BRAD), and preformed for bioinformatics analysis. The BocPDS1 and BocPDS2 genes mapped to chromosomes 3 and 4, and contains an open reading frame of 1,698 bp and 1,692bp that encodes a 565 and 563 amino acid protein, respectively. Subcellular localization predicted both BocPDS1 and BocPDS2 were in the chloroplast. The conserved domain of the BocPDS1 and BocPDS2 proteins is PLN02612. Homology analysis indicates that the levels of identity between BocPDS1 and BocPDS2 were 94%, and the PDS protein is apparently conserved during plant evolution, and BocPDSs are most closely related to Cruciferae plants. The findings of the present study provide a molecular basis for the elucidation of PDS gene function in cabbage.

Copyright

© 2018, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Bo Sun
AU  - Min Jiang
AU  - Shengling Xue
AU  - Aihong Zheng
AU  - Fen Zhang
AU  - Haoru Tang
PY  - 2018/08
DA  - 2018/08
TI  - Bioinformatics Analysis of the Phytoene Dehydrogenase Gene in Cabbage (Brassica Oleracea Var. Capitata)
BT  - Proceedings of the 2nd International Conference on Material Science, Energy and Environmental Engineering (MSEEE 2018)
PB  - Atlantis Press
SP  - 300
EP  - 305
SN  - 2352-5401
UR  - https://doi.org/10.2991/mseee-18.2018.54
DO  - 10.2991/mseee-18.2018.54
ID  - Sun2018/08
ER  -