Characterization of an exo-β-D-glucosaminidase from the hyperthermophilic archaeon Pyrococcus furiosus

DOI

10.2991/mmeceb-15.2016.52How to use a DOI?

Keywords

D-glucosamine; exo-β-D-glucosaminidase; archaeon; Pyrococcus furiosus

Abstract

D-glucosamine is an important chemical compound in industry. Exo-β-D-glucosaminidase (EC number: 3.2.1.165) is a potential enzyme which can be used in production of D-glucosamine. In this study, an exo-ß-D-glucosaminidase from the hyperthermophilic archaeon Pyrococcus furiosus was cloned and expressed in E. coli and purified to homogeneity. The therrnostable enzyme had a moleculat mass of 90 kDa and could hydrolyze chitosan or chitooligosaccharide to produce D-glucosamine. Moreover, thin layer chromatography showed that the enzyme could not act on lactose, indicating it is not a ß-galactosidase. The enzymatic properties of this exo-ß-D-glucosaminidase suggest its potential applications in biotechnology to produce D-glucosamine.

Copyright

© 2016, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Fei Wang
AU  - Bo Liu
AU  - Tanming Liu
PY  - 2015/12
DA  - 2015/12
TI  - Characterization of an exo-β-D-glucosaminidase from the hyperthermophilic archaeon Pyrococcus furiosus
BT  - Proceedings of the 2015 2nd International Conference on Machinery, Materials Engineering, Chemical Engineering and Biotechnology
PB  - Atlantis Press
SP  - 264
EP  - 267
SN  - 2352-5401
UR  - https://doi.org/10.2991/mmeceb-15.2016.52
DO  - 10.2991/mmeceb-15.2016.52
ID  - Wang2015/12
ER  -