Optimization of Arginine Fermentation Culture Medium on Recombinant Strain of Corynebacterium glutamicum

DOI

10.2991/mmebc-16.2016.66How to use a DOI?

Keywords

carbon; Nitrogen; Arginine; Fermentation

Abstract

In the recombinant strain Corynebacterium glutamicum ATCC14067-T18-argB, the key kinase (N-acetylglutamate kinase) was expressed with the PEC-T18mob2 plasmid. The ability of producing L-arginine was increased. Aimed at improving the production of L-arginine further, several factors include soya peptone, glucose, urea, calcium carbonate and liquid volume were studied. Based on the one-variable-at-a-time experiment of the fermentation medium, three main significant factors of the soya peptone, urea and glucose were screened by the Box-Bohnken design. After the optimization of fermentation medium by the Box-Bohnken results showed optimal medium containing of soya peptone 9.0 g/L, glucose 8.0 g/L and urea 8.0 g/L. The mutant ATCC14067-T18-argB could accumulate 10.77g/L L-arginine after 72h fermentation. Compared with the initial fermentation medium, the production on average increased nearly 3 times. The result of comparative experiment showed that the increase of urea which is the nitrogen and the decrease of glucose which is the carbon source made contribution to the increase of L-arginine.

Copyright

© 2016, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Download article (PDF)

TY  - CONF
AU  - Hongming Tian
PY  - 2016/06
DA  - 2016/06
TI  - Optimization of Arginine Fermentation Culture Medium on Recombinant Strain of Corynebacterium glutamicum
BT  - Proceedings of the 2016 6th International Conference on Machinery, Materials, Environment, Biotechnology and Computer
PB  - Atlantis Press
SP  - 321
EP  - 327
SN  - 2352-5401
UR  - https://doi.org/10.2991/mmebc-16.2016.66
DO  - 10.2991/mmebc-16.2016.66
ID  - Tian2016/06
ER  -