Proceedings of the 2016 2nd International Conference on Materials Engineering and Information Technology Applications (MEITA 2016)

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Modulation of Quorum-Regulatory sRNA on Various Physiological Functions and Virulence of Vibrio alginolyticus

Authors
Huan Liu, Jinfang Yang, Shanshan Zhang, Wang Liu, Xuefeng Chen
Corresponding Author
Huan Liu
Available Online February 2017.
DOI
10.2991/meita-16.2017.49How to use a DOI?
Keywords
Qrr1, Vibrio alginolyticus, Physiological function, Virulence.
Abstract

Vibrio alginolyticus is one of the important fish pathogen in China, bringing about serious economic loss to aquaculture. sRNA (Small non-coding RNA, sRNA) modulate the target genes on the post-transcriptional level with the aid of the chaperon protein, Hfq and play essential roles in the regulation of various physiological functions, like virulence. In this paper, a quorum regulatory sRNA, Qrr1, was screened and cloned. Besides, An in-frame deletion was made in qrr1 by allelic exchange, using the suicide plasmid. Meanwhile, the plasmid, pMMB206 was utilized to carry the contact qrr1 gene into the mutant strain qrr1 to generate the complemented strain, qrr1+. The roles of this gene in different physiological functions of Vibrio alginolyticus were characterized. The results demonstrated that Qrr1 obviously promoted the growth of Vibrio alginolyticus during exponential and stationary stage. Moreover, the ability of mobility was almost absent, the biofilm formation and extracellular protease production were all impaired in the qrr1 mutant strain, contrast to the wild-type strain, which means Qrr1 participates in the virulence regulation as an important regulator in Vibrio alginolyticus.

Copyright
© 2017, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 2016 2nd International Conference on Materials Engineering and Information Technology Applications (MEITA 2016)
Series
Advances in Engineering Research
Publication Date
February 2017
ISBN
978-94-6252-304-3
ISSN
2352-5401
DOI
10.2991/meita-16.2017.49How to use a DOI?
Copyright
© 2017, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

risenwbib
TY  - CONF
AU  - Huan Liu
AU  - Jinfang Yang
AU  - Shanshan Zhang
AU  - Wang Liu
AU  - Xuefeng Chen
PY  - 2017/02
DA  - 2017/02
TI  - Modulation of Quorum-Regulatory sRNA on Various Physiological Functions and Virulence of Vibrio alginolyticus
BT  - Proceedings of the 2016 2nd International Conference on Materials Engineering and Information Technology Applications (MEITA 2016)
PB  - Atlantis Press
SP  - 238
EP  - 243
SN  - 2352-5401
UR  - https://doi.org/10.2991/meita-16.2017.49
DO  - 10.2991/meita-16.2017.49
ID  - Liu2017/02
ER  -