Enzyme Catalyzed Synthesis of a, -fluoromethylene-ATP and Optimization of Reaction Conditions

DOI

10.2991/meic-14.2014.156How to use a DOI?

Keywords

; -fluoromethylene-ATP;Catalysis;NTP;PK;Optimized

Abstract

, -fluoromethylene-ATP is advantageously obtained via pho-sphorylation of corresponding ADP analogue using catalytic NTP(N=A or U),PEP,nucleoside diphosphate kinase(NDPK), and pyruvate kinase(PK). In order to find the optimized reaction conditions of synthesis of , -CHF -ATP via the catalysis of ATP or UTP , the effect of the ratio of PK/NDPK and the dosage of catalyst (ATP, UTP) on the reaction yield and reaction rate were investigated .the yield and reaction rate are discussed in relation to the proportion of PK/NDPK and the amount and types of NTP. via the catalysis of ATP, When the ratio of PK/NDPK is 3, the ratio of ATP / , -CHF -ADP is 5% the reaction conditions are optimized. But ,via the catalysis of UTP, When the ratio of PK/NDPK is 3, the reaction rate is small,so.,further optimization of reaction conditions is necessary; Via the catalysis of UTP, when the ratio of PK/NDPK is 3, the ratio of UTP / , -CHF -ADP is 30% ,the reaction conditions are optimized.

Copyright

© 2014, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Yan Li
AU  - Zhigang Zeng
AU  - Minghu Wu
AU  - Yanggen Hu
PY  - 2014/11
DA  - 2014/11
TI  - Enzyme Catalyzed Synthesis of a, -fluoromethylene-ATP and Optimization of Reaction Conditions
BT  - Proceedings of the 2014 International Conference on Mechatronics, Electronic, Industrial and Control Engineering
PB  - Atlantis Press
SP  - 698
EP  - 701
SN  - 2352-5401
UR  - https://doi.org/10.2991/meic-14.2014.156
DO  - 10.2991/meic-14.2014.156
ID  - Li2014/11
ER  -