Determination of Total Alkaloids in Different Parts of Actinidia arguta by Spectrophotography

DOI

10.2991/mebe-15.2015.31How to use a DOI?

Keywords

Actinidia arguta; Total Alkaloids; Spectrophotography

Abstract

Total Alkaloids are important active materials of Actinidia arguta. However, no study has been conducted to explore the content determination method of the Actinidia arguta total alkaloid. An effective method was established in order to determine the total alkaloids from different parts of Actinidia arguta. Using ultrasonic cleaner, the total alkaloids were extracted from the samples and tested at 415nm with the spectroscopy by the means of acid dye method. The calibration curve of aconitine were linear (r=0.9997), and the recovery rate was prefect (98.9%). The results of measurement were consistent in 3 hours. The content of alkaloids in roots was the highest, with 1.25mg/g, followed by leaves and fruits, the least were stems. The method is simple and effective, and the contents of total alkaloids were different in different parts of Actinidia arguta.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Yang-yang Liu
AU  - Chang-jiang Liu
PY  - 2015/04
DA  - 2015/04
TI  - Determination of Total Alkaloids in Different Parts of Actinidia arguta by Spectrophotography
BT  - Proceedings of the 2015 International Conference on Materials, Environmental and Biological Engineering
PB  - Atlantis Press
SP  - 127
EP  - 130
SN  - 2352-5401
UR  - https://doi.org/10.2991/mebe-15.2015.31
DO  - 10.2991/mebe-15.2015.31
ID  - Liu2015/04
ER  -