Research on the Effect of BMI1 Gene Silencing on the Proliferation Activity of Human Breast Cancer Cells

DOI

10.2991/mcei-18.2018.18How to use a DOI?

Keywords

BMI1 gene; Breast cancer cell; Gene silencing; Cell apoptosis; Cell proliferation

Abstract

Objectives: The purpose is to study the effect of proto oncogene BMI1(B-cell specific moloney murine leukemiavirus insertion site 1) gene silencing on the proliferation inhibition and 5-Fu sensitivity of human breast cancer cells through experiment in vitro, and to explore the feasibility of the combined treatment of breast cancer by BMI1 gene silencing and 5-Fu. Methods: The MTT method was used to detect the inhibitory effects of 5-Fu on the proliferation of MCF-7 cells, MCF-7/5-Fu cells and their BMI1 gene silencing cells. Morphological changes of cells in each group stained with acridine orange were observed by fluorescence. The flow cytometry was used to detect the effect of 5-Fu on the apoptosis rate and cell cycle of each group. Results: 5-Fu inhibited the growth of human breast cancer cells, and presented time - dose dependence. At 48h, the IC50 of cells in each group inhibited by 5-Fu was respectively: MCF-7(2.85±0.21), MCF-7/BMI1si (1.78±0.25), MCF-7/5-Fu(8.05±0.38) and MCF-7/5-Fu/BMI1si (5.65±0.27) μg/mL. BMI1 gene silencing cells were significantly lower than breast cancer cells(LSD-t=2.845，3.232；P<0.05). If 4μg/mL 5-Fu acts on the cells of each group for 48h, it can induce obvious cell apoptosis features in MCF-7/5-Fu/BMI1si cells. The apoptosis rate of BMI1 gene silencing group was significantly higher than that of breast cancer cells MCF-7 and MCF-7/5-Fu(LSD-t=7.826，8.231; P<0.05). The cell proportion of BMI1 gene silencing cells in G0/G1 phase also increased significantly (LSD-t=10.512，8.053; P<0.05). Conclusions: BMI1 gene silencing can enhance the sensitivity of breast cancer cells to 5-Fu and promote cell apoptosis.

Copyright

© 2018, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Download article (PDF)

TY  - CONF
AU  - Penghui Li
AU  - Dan Liu
AU  - Qingbu Mei
AU  - Xiuhua Wang
AU  - Yi Zheng
AU  - Hongming Pan
AU  - Lihong Zheng
PY  - 2018/06
DA  - 2018/06
TI  - Research on the Effect of BMI1 Gene Silencing on the Proliferation Activity of Human Breast Cancer Cells
BT  - Proceedings of the 2018 8th International Conference on Mechatronics, Computer and Education Informationization (MCEI 2018)
PB  - Atlantis Press
SP  - 88
EP  - 93
SN  - 2352-538X
UR  - https://doi.org/10.2991/mcei-18.2018.18
DO  - 10.2991/mcei-18.2018.18
ID  - Li2018/06
ER  -