Lipase–catalyzed Kinetic Resolution of Naproxen

DOI

10.2991/lemcs-15.2015.343How to use a DOI?

Keywords

Naproxen; Caria Papaya lipase; Asymmetric hydrolysis; Chiral separation; Enantiomeric ratio

Abstract

Naproxen is one of common non-steroidal anti-inflammatory drugs (NSAIDs). Its pharmacological properties vary with different configurations. In order to improve the drug efficacy, it is significant to prepare high enantiopure drugs. In this study, carica papaya lipase (CPL) and papain extracted from the crude papaya enzyme are used for the kinetic resolution of recemic Naproxen. The types of enzyme, the influences of reaction system, the amount of lipase and H2O were studied in order to obtain the optimum conditions. The results showed that carica papaya lipase has resolution ability over Naproxen, but papain has no resolution ability over Naproxen. In the microaqueous-isooctane system (1.2ml H2O, 30ml isooctane, the concentration of Naproxen methyl ester is 10mg/ml), 1g CPL was used to catalyze the reaction. The reactions were carried out in a shaker (180r/min) at 30 . In the condition described above, conversion of substrate nears 30% after 10d, and the enantiomeric ratio (E) is 30..

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Lirui Sun
AU  - Jianbo Cheng
AU  - Hongyue Sun
AU  - Shuai Liu
AU  - Jiaying Xin
PY  - 2015/07
DA  - 2015/07
TI  - Lipase–catalyzed Kinetic Resolution of Naproxen
BT  - Proceedings of the International Conference on Logistics, Engineering, Management and Computer Science
PB  - Atlantis Press
SP  - 1696
EP  - 1699
SN  - 1951-6851
UR  - https://doi.org/10.2991/lemcs-15.2015.343
DO  - 10.2991/lemcs-15.2015.343
ID  - Sun2015/07
ER  -