Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)

Genetic Variation of Black Capped White Eye Zosterops atricapilla (Aves: Zosteropidae) Based on Mitochondrial DNA Cytochrome B Gene

Authors
Deby Permana, Jarulis, Sipriyadi, Santi Nurul Kamilah
Corresponding Author
Jarulis
Available Online 23 June 2021.
DOI
10.2991/absr.k.210621.058How to use a DOI?
Keywords
Cytb, Conservation, illegal trafficking, mtDNA, Zosteropidae
Abstract

The purpose of this research was to explore the genetic variation of Black Capped White eye Zosterops articapilla using the mitochondrial DNA cytochrome b gene. The blood sample was collected via pectoral vein of bird from Panorama markets, Bengkulu city. DNA genome were isolated and purified from the blood following DNeasy protocol® Blood and Tissue Kit cat no. 69504 (50), based on Qiagen’s Spin-Column Protocol procedure. We used polymerase chain reaction machine for amplification DNA template with specific primer (ZCYTBF and ZCYTBR). The results showed that length of cyt b gene sequence (n=6) was 725 bp and had 717 bp (98.89%) conservative site. The average of intraspecific genetic distance was 0.04, interspecific 0.063, and between Indonesian and overseas (outgroup) was 0.138.

Copyright
© 2021, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)
Series
Advances in Biological Sciences Research
Publication Date
23 June 2021
ISBN
978-94-6239-399-8
ISSN
2468-5747
DOI
10.2991/absr.k.210621.058How to use a DOI?
Copyright
© 2021, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Deby Permana
AU  - Jarulis
AU  - Sipriyadi
AU  - Santi Nurul Kamilah
PY  - 2021
DA  - 2021/06/23
TI  - Genetic Variation of Black Capped White Eye Zosterops atricapilla (Aves: Zosteropidae) Based on Mitochondrial DNA Cytochrome B Gene
BT  - Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)
PB  - Atlantis Press
SP  - 340
EP  - 345
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.210621.058
DO  - 10.2991/absr.k.210621.058
ID  - Permana2021
ER  -