Effect of Honey Supplementation on Sperm Abnormality in Sperm Preservation of Brek Fish Systomous orphoides
- DOI
- 10.2991/absr.k.210621.054How to use a DOI?
- Keywords
- Sperm abnormality, honey, short-term preservation, Systomus orphoides
- Abstract
Honey is consisting of simple sugar, such as glucose, sucrose, and fructose, that could serve as a source of nutrition for sperm cells during preservation. The purpose of the study was to assess the effect of honey supplementation in the extender on the spermatozoa abnormality of brek fish Systomus orphoides (Valenciennes, 1842) 48 h after preserved at 4 °C. The brek fish semen was obtained using the hand stripping method, and the diluent used is a mixture of fish ringers, 10% Methanol, and different concentrations of Honey solution (5%; 10%; 15%; and 20%). Furthermore, equilibration was carried out at 28 °C for 1 minutes, and the sperm storage was carried out at 4 °C for 48 h. The evaluation of semen was carried out macroscopically (color, volume, and pH) and microscopically (abnormalities). The results of the ANOVA test followed by the Tukey test was significantly different (p <0.05) in the abnormality. The results showed that treatment of 5% honey solution was the optimum concentration because the lowest abnormality was (11.85±0.45%).
- Copyright
- © 2021, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Sri Widiyanti Rahayu Hilia AU - Abinawanto AU - Anang Hari Kristanto PY - 2021 DA - 2021/06/23 TI - Effect of Honey Supplementation on Sperm Abnormality in Sperm Preservation of Brek Fish Systomous orphoides BT - Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020) PB - Atlantis Press SP - 319 EP - 323 SN - 2468-5747 UR - https://doi.org/10.2991/absr.k.210621.054 DO - 10.2991/absr.k.210621.054 ID - Hilia2021 ER -