Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)

Effect of Honey Supplementation on Sperm Abnormality in Sperm Preservation of Brek Fish Systomous orphoides

Authors
Sri Widiyanti Rahayu Hilia, Abinawanto, Anang Hari Kristanto
Corresponding Author
Sri Widiyanti Rahayu Hilia
Available Online 23 June 2021.
DOI
10.2991/absr.k.210621.054How to use a DOI?
Keywords
Sperm abnormality, honey, short-term preservation, Systomus orphoides
Abstract

Honey is consisting of simple sugar, such as glucose, sucrose, and fructose, that could serve as a source of nutrition for sperm cells during preservation. The purpose of the study was to assess the effect of honey supplementation in the extender on the spermatozoa abnormality of brek fish Systomus orphoides (Valenciennes, 1842) 48 h after preserved at 4 °C. The brek fish semen was obtained using the hand stripping method, and the diluent used is a mixture of fish ringers, 10% Methanol, and different concentrations of Honey solution (5%; 10%; 15%; and 20%). Furthermore, equilibration was carried out at 28 °C for 1 minutes, and the sperm storage was carried out at 4 °C for 48 h. The evaluation of semen was carried out macroscopically (color, volume, and pH) and microscopically (abnormalities). The results of the ANOVA test followed by the Tukey test was significantly different (p <0.05) in the abnormality. The results showed that treatment of 5% honey solution was the optimum concentration because the lowest abnormality was (11.85±0.45%).

Copyright
© 2021, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)
Series
Advances in Biological Sciences Research
Publication Date
23 June 2021
ISBN
978-94-6239-399-8
ISSN
2468-5747
DOI
10.2991/absr.k.210621.054How to use a DOI?
Copyright
© 2021, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Sri Widiyanti Rahayu Hilia
AU  - Abinawanto
AU  - Anang Hari Kristanto
PY  - 2021
DA  - 2021/06/23
TI  - Effect of Honey Supplementation on Sperm Abnormality in Sperm Preservation of Brek Fish Systomous orphoides
BT  - Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)
PB  - Atlantis Press
SP  - 319
EP  - 323
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.210621.054
DO  - 10.2991/absr.k.210621.054
ID  - Hilia2021
ER  -