Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)

Elicitor Effect of Chitosan on In Vitro Culture of Different Explants of Physalis Accessions From East Java

Authors
Retno Mastuti, Jati Batoro, Budi Waluyo
Corresponding Author
Retno Mastuti
Available Online 23 June 2021.
DOI
10.2991/absr.k.210621.064How to use a DOI?
Keywords
accession, chitosan, elicitors, Madura, Physalis
Abstract

Chitosan is a natural molecule derived from arthropods and fungi that can stimulate many physiological responses in plants. Chitosan had also shown positive effect on in vitro shoot regeneration. Therefore, this research aims to observe the response of shoot growth of in vitro Physalis plant accessions obtained from several areas in Madura Island and East Java on chitosan effect. Two types of explants shoot tips and in vitro nodal explants for each Physalis plant accession were cultured on elicitation medium (MS basal medium supplemented with BAP 2 mg / l and IAA 0.05 mg /l) containing chitosan elicitor (0, 75, 125 mg /l). The number and height of shoots were observed weekly for six weeks. The results showed that both types of explants from all accessions were able to regenerate and multiply shoots however shoot tip explants showed better growth. A2 accessions from Madura Sampang was able to regenerate shoots in high a number than other accessions on chitosan concentration of 125 mg / l. These results provide a future prospect to study synthesis of secondary metabolites in Physalis accession induced by chitosan elicitors.

Copyright
© 2021, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)
Series
Advances in Biological Sciences Research
Publication Date
23 June 2021
ISBN
978-94-6239-399-8
ISSN
2468-5747
DOI
10.2991/absr.k.210621.064How to use a DOI?
Copyright
© 2021, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Retno Mastuti
AU  - Jati Batoro
AU  - Budi Waluyo
PY  - 2021
DA  - 2021/06/23
TI  - Elicitor Effect of Chitosan on In Vitro Culture of Different Explants of Physalis Accessions From East Java
BT  - Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)
PB  - Atlantis Press
SP  - 382
EP  - 386
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.210621.064
DO  - 10.2991/absr.k.210621.064
ID  - Mastuti2021
ER  -