Identification and Antibiotic Resistance Profile of Bacteria From Fruit Bat (Chironax melanocephalus)

DOI

10.2991/absr.k.210621.081How to use a DOI?

Keywords

Antibiotic resistance, identification, bacteria, wildlife

Abstract

Antibiotic resistance is one of the most serious health issues in the world which is commonly attributed to the overuse of antibiotics whether in human or animal treatment. The dispersion of antibiotic resistance genes not only found in bacteria from the clinical sample but also found in wildlife animals. Therefore, the aim of this research is to isolate, identify and perform antibiotic resistance assay in bacterial isolates from fruit bat Chironax melanocephalus. Animal samples were taken by using a mist net installed in one of the spots in Gunung Halimun Salak National Park (TNGHS). Bacteria sample was taken using an oral and rectal swab. The bacteria were then isolated in the laboratory by using xylose lactose deoxycholate (XLD) agar medium. There were two bacteria successfully isolated and identified using 16s rDNA amplification. The identification was performed by comparing the 16s rDNA genes to the two online databases i.e BLAST-N in Genebank and 16s-Based ID in the EzBioCloud database. According to the identification results, isolate 4A.1 was identified as Proteus mirabilis while isolate 5.B.2 is identified as Serratia marcescens. Then antibiotic susceptibility test was performed using the disk diffusion method using four different antibiotics disk i.e oxacillin, cefoxitin, amikacin and amoxicillin-clavulanic acid. The resistance profile of each bacteria is different between P. mirabilis and S. marcescens in each antibiotic. However, all isolates were found susceptible against amikacin 30 µg.

Copyright

© 2021, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Masrukhin
AU  - Sugiyono Saputra
AU  - Syaiful Rizal
AU  - Pangda Sopha Sushadi
AU  - Nanang Supriatna
PY  - 2021
DA  - 2021/06/23
TI  - Identification and Antibiotic Resistance Profile of Bacteria From Fruit Bat (Chironax melanocephalus)
BT  - Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)
PB  - Atlantis Press
SP  - 478
EP  - 484
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.210621.081
DO  - 10.2991/absr.k.210621.081
ID  - 2021
ER  -