Antiacne Compound from the Methanolic Extract of Hyptis (Hyptis capitata) Roots

DOI

10.2991/absr.k.210408.045How to use a DOI?

Keywords

Antiacnce, Methanolic extract, Hyptis capitata, Maceration

Abstract

In the search of the antiacne agent from tropical medicinal plants, the methanolic extract of Hyptis capitata root was subjected to antiacne assay guided isolation of active compound. Extraction was conducted by maceration using methanol. The methanol extract was separated by column chromatography assisted by thin-layer chromatography to obtain active compounds. Antiacne activity against Propionibacterium acnes was analyzed using agar diffusion method at a concentration of 200 μg/well with chloramphenicol as a positive control. Column chromatography of methanol extract gave five active fractions, in which fractions AH1 and AH2 to be the strongest by 85% and 82% inhibition respectively. Fraction AH1 was then chromatographed on silica gel column to obtain 16 subfractions. The active fractions isolated from H. capitata have the potential to be developed as a natural antiacne. Further purification, identification, and characterization of the active compounds would be our priority in future studies.

Copyright

© 2021, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Rahmini
AU  - Irawan Wijaya Kusuma
AU  - Cita Tri Murni Andayati
AU  - Dedi Satria
AU  - Yhiya Amen
AU  - Kuniyoshi Shimizu
PY  - 2021
DA  - 2021/04/10
TI  - Antiacne Compound from the Methanolic Extract of Hyptis (Hyptis capitata) Roots
BT  - Proceedings of the Joint Symposium on Tropical Studies (JSTS-19)
PB  - Atlantis Press
SP  - 274
EP  - 276
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.210408.045
DO  - 10.2991/absr.k.210408.045
ID  - 2021
ER  -