Antibacterial Activity Against Propionibacterium acnes of n-Hexane Fractions from Siam Weed Leaves (Chromolaena odorata)

DOI

10.2991/absr.k.210408.033How to use a DOI?

Keywords

Antiacne, Phytochemical, Fractions, Chromolaena odorata

Abstract

The present study aims to separate the active fractions from the n-hexane extract of Siam weed (Chromolaena odorata (L) R.M. King & H. Rob) leaves with potential as the antiacne agent. The present work includes isolation of active fractions guided by antiacne assay against Propionibacterium acnes and phytochemical analysis of the active fractions. Extraction was conducted by maceration using n-hexane. An agar well diffusion assay was applied to evaluate the antiacne activity of the extract at the concentration of 400 μg/well with chloramphenicol as a positive control. Column chromatography assisted by thin-layer chromatography of n-hexane extract gave six active fractions. Fractions TH5 and TH6 displayed the highest inhibition of 51% and 47% respectively. Further purification of the fraction yielded more than 14 subfractions. Phytochemical analysis indicated that fraction TH5 contains alkaloid and steroid while fraction TH6 contains alkaloid. The active fractions isolated from C. odorata have the potential to be developed as a natural antiacne.

Copyright

© 2021, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Irawan Wijaya Kusuma
AU  - Rahmini
AU  - Enih Rosamah
AU  - Supomo
AU  - Harlinda Kuspradini
PY  - 2021
DA  - 2021/04/10
TI  - Antibacterial Activity Against Propionibacterium acnes of n-Hexane Fractions from Siam Weed Leaves (Chromolaena odorata)
BT  - Proceedings of the Joint Symposium on Tropical Studies (JSTS-19)
PB  - Atlantis Press
SP  - 201
EP  - 204
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.210408.033
DO  - 10.2991/absr.k.210408.033
ID  - Kusuma2021
ER  -