Establishment of Bimolecular Complementation System for the Interaction Detection between Epstein-barr Virus Nuclear Antigen 1 and Host Proteins

DOI

10.2991/itms-15.2015.253How to use a DOI?

Keywords

Bimolecular complementation; Epstein-Barr virus nuclear antigen 1; Protein-protein interaction

Abstract

Epstein-Barr virus nuclear antigen 1 (EBNA1) is essential for EBV episome’s replication and mitotic segregation. During this process, EBNA1 may interact with a lot of host factors. Bimolecular complementation (BiMC) assay is a useful tool for a detection of protein-protein interaction in living cells. In the present study, a plasmid for expressing a fusion protein CYFP-EBNA1 was constructed. As expected, it could express CYFP-EBNA1 and localize in the cellular nuclear. The result also demonstrated that the plasmid is allowed to be used in the BiMC system.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Lielian Zuo
AU  - Meijuan Zhu
AU  - Shujuan Du
AU  - Qijia Yan
AU  - Haibo Yuan
AU  - Jing Yang
AU  - Guiyuan Li
AU  - Jianhong Lu
AU  - Liming He
PY  - 2015/11
DA  - 2015/11
TI  - Establishment of Bimolecular Complementation System for the Interaction Detection between Epstein-barr Virus Nuclear Antigen 1 and Host Proteins
BT  - Proceedings of the 2015 International Conference on Industrial Technology and Management Science
PB  - Atlantis Press
SP  - 1047
EP  - 1050
SN  - 2352-538X
UR  - https://doi.org/10.2991/itms-15.2015.253
DO  - 10.2991/itms-15.2015.253
ID  - Zuo2015/11
ER  -