Soy Protein Isolate (SPI) Based Delivery System as Promising Mastitis Vaccine Carrier Candidate

Dadang Priyoatmojo; Tri Handayani; Afi Candra Trinugraha; Teguh Wahyono; Nina Herlina

doi:10.2991/absr.k.220309.035

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Soy Protein Isolate (SPI) Based Delivery System as Promising Mastitis Vaccine Carrier Candidate

Authors

Dadang Priyoatmojo¹^{, *}, Tri Handayani¹, Afi Candra Trinugraha¹, Teguh Wahyono¹, Nina Herlina²

¹Department of Agriculture, Research and Technology Center for Isotopes and Radiation Application (PRTAIR), National Research and Innovation Agency of Indonesia (BRIN) Jakarta, Indonesia

²Research Center for Biotechnology BRIN, Cibinong Bogor West Java, Indonesia

^*Corresponding author. Email: dadangpr@batan.go.id

Corresponding Author

Dadang Priyoatmojo

Available Online 29 March 2022.

DOI: 10.2991/absr.k.220309.035 How to use a DOI?
Keywords: Loading Efficiency; Loading Capacity; Soy protein isolates; Vaccine Carrier
Abstract: An orally administered vaccine application is a part of strategies on veterinary vaccine development. Soy protein isolate (SPI) has been scientifically tested in various studies as a potent drug carrier. Mastitis is one of the livestock diseases mainly caused by Streptococcus agalactiae, which can be controlled through vaccination programs. This study aimed to determine loading efficiency (LE) and loading capacity (LC) in various formulations of SPI, sodium alginate loaded with inactivated group B streptococcus trehalose (GBS-trehalose) combination as oral vaccine carrier candidates. Based on the SPI solubility test, we obtained a high percentage of solubility at pH 6-10, ranging from 83.40% to 99.39%. The prepared GBS-trehalose loaded SPI-alginate microparticles (pH 6) with a ratio of 1.03:1 yielded LE of 47.37 % and 3.79% LC. The lower concentration of GBS-trehalose loaded to SPI-alginate with ratios 0.876:1 and 0.657:1 yields a higher LE of 70.77% to 92.08% and 6.66% to 10.52% LC, confirming the valuable microencapsulation properties of SPI-alginate and the suitability of the ratio formulations. These results demonstrate that SPI-alginate is relevant for developing an inactivated bacterial vaccine carrier for the oral route. More research is needed for microparticles characterization, release capability and in vitro stability under various environmental conditions.
Copyright: © 2022 The Authors. Published by Atlantis Press International B.V.
Open Access: This is an open access article under the CC BY-NC license.

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Volume Title: Proceedings of the International Conference on Improving Tropical Animal Production for Food Security (ITAPS 2021)
Series: Advances in Biological Sciences Research
Publication Date: 29 March 2022
ISBN: 978-94-6239-557-2
ISSN: 2468-5747
DOI: 10.2991/absr.k.220309.035 How to use a DOI?
Open Access: This is an open access article under the CC BY-NC license.

Cite this article

ris enw bib

TY  - CONF
AU  - Dadang Priyoatmojo
AU  - Tri Handayani
AU  - Afi Candra Trinugraha
AU  - Teguh Wahyono
AU  - Nina Herlina
PY  - 2022
DA  - 2022/03/29
TI  - Soy Protein Isolate (SPI) Based Delivery System as Promising Mastitis Vaccine Carrier Candidate
BT  - Proceedings of the International Conference on Improving Tropical Animal Production for Food Security (ITAPS 2021)
PB  - Atlantis Press
SP  - 169
EP  - 174
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220309.035
DO  - 10.2991/absr.k.220309.035
ID  - Priyoatmojo2022
ER  -

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