A Microarray Image Gridding Method Based on Projection Transformation and Power Spectral Analysis

DOI

10.2991/isci-15.2015.7How to use a DOI?

Keywords

Gene Chip; Microarray Image Gridding; Projection Transformation; Power Spectral Analysis

Abstract

Microarray image gridding is one important step of microarray image analysis to determine 2D image coordinates of all array spots in the hybridized gene chip image. Accuracy of image gridding will affect the reliability of gene-chip data extraction and even the final analysis results of gene-chip assays. To promote microarray image gridding accuracy and computation efficiency, we presented a microarray image gridding method based on image projection transformation and power spectral analysis. Firstly we transformed 2D microarray image into vertical and horizontal 1D projection sequences, secondly utilized signal processing methods of low pass filtering, zero mean, FFT, and power spectral estimation by periodogram method to get spots array row and column span information, and finally realized the microarray image gridding according to the local maxima and span information of spots array. The gridding experiments showed that this method had met the requirements of computing accuracy and efficiency of microarray image gridding.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Yanjun Feng
AU  - Kai Song
AU  - Jun Liu
PY  - 2015/01
DA  - 2015/01
TI  - A Microarray Image Gridding Method Based on Projection Transformation and Power Spectral Analysis
BT  - Proceedings of the 2015 International Symposium on Computers & Informatics
PB  - Atlantis Press
SP  - 44
EP  - 51
SN  - 2352-538X
UR  - https://doi.org/10.2991/isci-15.2015.7
DO  - 10.2991/isci-15.2015.7
ID  - Feng2015/01
ER  -