Screening and Culture Condition of Lipase Strains from Biodiesel

DOI

10.2991/ipemec-15.2015.222How to use a DOI?

Keywords

Biodiesel; isolation; separation; purification

Abstract

Biodiesel, a biodegradable, sustainable and clean energy has attracted growing interest in recent years, mainly due to development in bio-diesel fuel and ecological pressures including climatic changes. In this study, lipase strains FS14 extracted from soil which is polluted by oil and fat were analyzed .The highest enzyme activity strain was screened in culture of bromcresol purple. It was gram positive and short rod. The result of identification shows that it respectively belongs to Serratia sp. through the shape of the colony, determination physiology and biochemistry. The optimal culture conditions were determined as follows: initial pH 7.0 , at olive oil concentration of 8 to10 mg/L, incubation at 30 for 3d.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Chaocheng Zheng
AU  - Xinren You
PY  - 2015/05
DA  - 2015/05
TI  - Screening and Culture Condition of Lipase Strains from Biodiesel
BT  - Proceedings of the 2015 International Power, Electronics and Materials Engineering Conference
PB  - Atlantis Press
SP  - 1192
EP  - 1195
SN  - 2352-5401
UR  - https://doi.org/10.2991/ipemec-15.2015.222
DO  - 10.2991/ipemec-15.2015.222
ID  - Zheng2015/05
ER  -