Effect of Cocoa Pod Extract (Theobroma cacao L.) On Gout Levels of Mice (Mus musculus) Hypercholesterolemia Diabetes Mellitus Type 2 Model Through Enzyme Linked Immunosorbent Assay Reader Examination

DOI

10.2991/978-94-6463-392-4_14How to use a DOI?

Keywords

Uric Acid; Cocoa Fruit; High Fat Feed; Streptozotocin; Theobroma cacao

Abstract

Hyperuricemia is a condition that describes an increase in uric acid levels in the body, increased uric acid levels in the blood beyond normal limits will cause pain or tenderness. Cocoa pod skin contains a number of antioxidants such as flavonoids, alkaloids, polyphenols and tannins which can be used as an alternative treatment for hyperuricemia. This study aimed to determine the effect of cocoa pod extract on mice’s uric acid levels and determine the optimal dose of cocoa pod extract. This research was a quasi-experimental study with a pretest-posttest control group design approach with the independent variable of cocoa pod extract and the dependent variable of mice uric acid levels. Type 2 Diabetes Mellitus in this study was induced using 40 mg/kg BW streptozotocin and hypercholesterolemia was induced with a high-fat diet which was then measured using an ELISA reader. Data were analyzed using a paired t-test and One Way ANOVA test. Average uric acid levels between the control group and the 50 mg/kg BW extract, the 100 mg/kg BW group and the 150 mg/kg BW extract group were not significantly different (p > 0.05). The optimal dose of cocoa pod extract to reduce uric acid levels in mice is a dose of 150 mg/kg BW. Cacao pod extract affects the uric acid levels of mice at a dose of 50 mg/kg, 100 mg/kg, and 150 mg/kg. The mean levels of uric acid between the control group and the 50 mg/kg BW extract, the 100 mg/kg BW group and the 150 mg/kg BW extract group were not significantly different (p > 0.05).

Copyright

© 2024 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - I Putu Sudayasa
AU  - M. Rilan Ampurama Ruslan
AU  - Gita Julianti Malik
AU  - Muhamad Reza Iskandar
PY  - 2024
DA  - 2024/04/22
TI  - Effect of Cocoa Pod Extract (Theobroma cacao L.) On Gout Levels of Mice (Mus musculus) Hypercholesterolemia Diabetes Mellitus Type 2 Model Through Enzyme Linked Immunosorbent Assay Reader Examination
BT  - Proceedings of the 1st International Conference Medical and Health Science Halu Oleo (IMHO 2023)
PB  - Atlantis Press
SP  - 120
EP  - 126
SN  - 2468-5739
UR  - https://doi.org/10.2991/978-94-6463-392-4_14
DO  - 10.2991/978-94-6463-392-4_14
ID  - Sudayasa2024
ER  -