Utilization of Liquid Organic Waste as a Formulation of Biopesticide with Bacillus sp. DB12 for Controlling Fusarium oxysporum f.sp. cepae

DOI

10.2991/978-94-6463-334-4_34How to use a DOI?

Keywords

formulas; carrier material; Bacillus sp DB12; organic wastewater

Abstract

Synthetic (inorganic) carrier materials are expensive and their use is very limited, so they become an obstacle for the development of biopesticide formulas made from active biocontrol agents. Therefore, formula innovation technology is needed that can reduce research costs through the use of alternative carriers of liquid organic matter that are cheap and easy to obtain but still effective in controlling plant pathogens. The purpose of this study was to determine the right combination of liquid carrier formulas in maintaining population density and inhibition of Bacillus sp. DB12 against the pathogenic fungus Fusarium oxysporum f.sp. Cepae in-vitro as a basis for the manufacture of biological biopesticides. This study was designed in a completely randomized design (CRD) using 9 treatments of carrier composition with the addition of the biological agent Bacillus sp DB12. The waste used is tofu liquid waste (LCT), coconut liquid waste (LCK), and rice washing liquid waste (LCB). The treatment is as follows LCT 100% (L1), LCT 75% + LCK 25% (L2), LCT 50% + LCK 50% (L3), LCT 25% + LCK 75% (L4), LCK 100% (L5), 75% LCT + 25% LCB (L6), 50% LCT + 50% LCB (L7), 25% LCT + 75% LCB (L8), and 100% LCB (L9). The results showed that the combination of 75% LCT + 25% LCK carrier was able to provide the highest population density and inhibition after being stored for 12 weeks, and was significantly different from all carriers except 50% LCT + 50% LCK carrier. The antifungal activity ability of Bacillus sp DB12 from 75% LCT + 25% LCK carrier was categorized as strong based on the inhibition area.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Asrul
AU  - Rosmini
AU  - Seftian Layukan
AU  - Nurafifah
AU  - Lilis Suryani
AU  - Mifda
PY  - 2024
DA  - 2024/01/24
TI  - Utilization of Liquid Organic Waste as a Formulation of Biopesticide with Bacillus sp. DB12 for Controlling Fusarium oxysporum f.sp. cepae
BT  - Proceedings of the 2nd International Interdisciplinary Conference on Environmental Sciences and Sustainable Developments 2022 Environment and Sustainable Development (IICESSD-ESD 2022)
PB  - Atlantis Press
SP  - 199
EP  - 204
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-334-4_34
DO  - 10.2991/978-94-6463-334-4_34
ID  - 2024
ER  -