Comparision and evaluation of two pretreatments of different mechanisms for multi-Fusarium toxins

DOI

10.2991/ifmca-16.2017.134How to use a DOI?

Keywords

Immunoaffinity columns, MycoSep®226 multifunction purifying columns, Fusarium toxins, flour , liquid chromatography mass spectrometryzz (LC-MS/MS)

Abstract

A simultaneous determination method for four kinds of Fusarium toxins (zearalenone, deoxynivalenol, T-2 and HT-2 toxins) in flour was established using high performance liquid chromatography-tendem mass spectrometry (LC-MS/MS). Samples were cleaned up with MycoSep®226 multifunction purifying columns and immunoaffinity columns DZT MS-PREP separately. The recovery of the Mycosep® 226 multifunction purifying column is 69.42%~111.12%, and the relative standard deviation is 1.46%~13.24%. The recovery of the immunoaffinity columns DZT MS-PREP is 88.01%~107.31%, and the relative standard deviation is 1.09%~14.42%. 7 kinds of flour samples were determined, most of the detection results of the immunoaffinity columns DZT MS-PREP were higher than that of the Mycosep® 226 multifunction purifying column. The immunoaffinity columns provides an alternative effective and fast tool for simultaneous extraction of the residues of four Fusariumtoxins from real samples.

Copyright

© 2017, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Jia-Ning Zhang
AU  - Ke Ding
AU  - Xiang-Ning Chen
AU  - Tao Han
PY  - 2017/03
DA  - 2017/03
TI  - Comparision and evaluation of two pretreatments of different mechanisms for multi-Fusarium toxins
BT  - Proceedings of the 2016 International Forum on Mechanical, Control and Automation (IFMCA 2016)
PB  - Atlantis Press
SP  - 855
EP  - 863
SN  - 2352-5401
UR  - https://doi.org/10.2991/ifmca-16.2017.134
DO  - 10.2991/ifmca-16.2017.134
ID  - Zhang2017/03
ER  -