Proceedings of the 2016 International Forum on Energy, Environment and Sustainable Development

The excision of N terminal two amino acids of a novel human parathyroid hormone analog with dipeptidylpeptidase

Authors
Jiao Feng, Yan Zhu, Shuang Gao, Lihua Sun, Shuyan Ren, Tian Hong
Corresponding Author
Jiao Feng
Available Online May 2016.
DOI
10.2991/ifeesd-16.2016.98How to use a DOI?
Keywords
human Parathyroid hormone, analog, dipeptidyl peptidase, enzymatic reaction, prodrug.
Abstract

Pro-Pro-[Arg11]hPTH(1-34)-Pro-Pro-Asp (hPTH') is more effective than hPTH(1-34) in the treatment of osteoporosis in our previous studies. The hypothesis that the active form of hPTH' was introduced by the excision with dipeptidyl peptidase IV (DPPIV) in vivo was validated in the present study. Following the determinations of DPPIV activities in rats tissues and serum, the N-terminus dipeptides of hPTH' were excised by DPPIV in rats tissues and serum and the excision production was determined by Tris-Tricine-SDS-PAGE. The results indicated that the N-terminus dipeptides of hPTH' could be excised with DPPIV in rats tissues and serum. The molecular weight of excision production was greater than that of hPTH (1-34) and less than that of hPTH'. In addition, the excision production was injected to mice for detecting its effect on serum calcium of mice. The results suggested that the serum calcium levels of mice treated with hPTH' were similar with those of mice treated with hPTH (1-34). In conclusion, appearing to function as a prodrug, the active form of hPTH' was introduced by the excision with DPPIV in vivo.

Copyright
© 2016, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 2016 International Forum on Energy, Environment and Sustainable Development
Series
Advances in Engineering Research
Publication Date
May 2016
ISBN
978-94-6252-204-6
ISSN
2352-5401
DOI
10.2991/ifeesd-16.2016.98How to use a DOI?
Copyright
© 2016, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Jiao Feng
AU  - Yan Zhu
AU  - Shuang Gao
AU  - Lihua Sun
AU  - Shuyan Ren
AU  - Tian Hong
PY  - 2016/05
DA  - 2016/05
TI  - The excision of N terminal two amino acids of a novel human parathyroid hormone analog with dipeptidylpeptidase
BT  - Proceedings of the 2016 International Forum on Energy, Environment and Sustainable Development
PB  - Atlantis Press
SP  - 545
EP  - 549
SN  - 2352-5401
UR  - https://doi.org/10.2991/ifeesd-16.2016.98
DO  - 10.2991/ifeesd-16.2016.98
ID  - Feng2016/05
ER  -