Assessment of Phage Abundance in Water Samples of Napahai Wetland by Epifluorescence Microscopy

DOI

10.2991/iea-15.2015.168How to use a DOI?

Keywords

Napahai wetland; phage abundance; water samples; epifluorescene microscopy.

Abstract

Bacteriophage is abundant in various aquatic environment. Napahai is a seasonal plateau wetland located in Yunnan of China. But the reports on phage abundance of Napahai have not been seen. The object of this study was to make an accurate estimation for phage abundance of Napahai. We collected water samples at different sampling sites in December 2013 and detected the abundance by epifluorescence microscopy using SYBR green I or SYBR Gold. The results indicate that the phage abundance of water samples in Napahai wetland ranged from 1.35 to 3.54×109 virus-like particles L-1. The dying effect of SYBR Gold is better than SYBR green I. Phage abundance of Napahai is close to that of Yangtze River and higher than the Yunnan Plateau lake, Bita Lake (5×108 particles L-1) and largely lower than the polar lake Beaver Lake (2×1010 particles L-1) [1].

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - S. Li
AU  - H.Y. Yu
AU  - C. Sun
AU  - J.K. Li
AU  - Y.L. Wei
AU  - X.L. Ji
PY  - 2015/09
DA  - 2015/09
TI  - Assessment of Phage Abundance in Water Samples of Napahai Wetland by Epifluorescence Microscopy
BT  - Proceedings of the AASRI International Conference on Industrial Electronics and Applications (2015)
PB  - Atlantis Press
SP  - 675
EP  - 678
SN  - 2352-5401
UR  - https://doi.org/10.2991/iea-15.2015.168
DO  - 10.2991/iea-15.2015.168
ID  - Li2015/09
ER  -