Inhibitory Kinetics of 2'-Hydroxy-4'-methoxyacetophenone on Tyrosinase-catalyzing Reaction

DOI

10.2991/icsmim-15.2016.117How to use a DOI?

Keywords

tyrosinase. 2'-hydroxy-4'-methoxyacetophenone. inhibitory effect. Kinetics

Abstract

The inhibitory effects of 2'-hydroxy-4'-methoxyacetophenone on the activity diphenolase contained in tyrosinase was studied using enzymological kinetic method. 2'-hydroxy-4'-methoxyacetophenone was found to inhibit diphenolase activity of tyrosinase. The 2'-hydroxy-4'-methoxyacetophenone concentration leading to 50 % activity lost (IC50) was 0.60 mmol/L for diphenolase activity. The inhibition kinetics analyzed by Lineweaver-Burk plots found 2'-hydroxy-4'-methoxyacetophenone to be a mixed inhibitor for the oxidation of L-DOPA, the equilibrium constants for inhibitor binding with free enzyme (E), KI, and with enzyme-substract (ES) complex, IS, were 0.08mmol•L 1 and 0.12 mmol•L 1 respectively.

Copyright

© 2016, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Shengzhao Gong
AU  - Qingsheng Chen
AU  - Mengyi Xu
PY  - 2016/01
DA  - 2016/01
TI  - Inhibitory Kinetics of 2'-Hydroxy-4'-methoxyacetophenone on Tyrosinase-catalyzing Reaction
BT  - Proceedings of the 2015 4th International Conference on Sensors, Measurement and Intelligent Materials
PB  - Atlantis Press
SP  - 630
EP  - 634
SN  - 2352-538X
UR  - https://doi.org/10.2991/icsmim-15.2016.117
DO  - 10.2991/icsmim-15.2016.117
ID  - Gong2016/01
ER  -