A Type of Microorganism That Can Fix Greenhouse Gas (CO2 and N2 )Simultaneously-Isolation and Validation Experiment of Facultative CO2 and N2-fixing Bacteria

DOI

10.2991/icseee-16.2016.78How to use a DOI?

Keywords

facultative CO2 and N2-fixing bacteria, carbon and nitrogen-free medium, carbon fixation enzymes, nitrogenase.

Abstract

The N2-fixing microbial azotobacter and the CO2-fixing bacteria have been discovered and researched. However, few has been reported so far that can fix CO2, N2 simultaneously, while taking CO2, N2 as microbial carbon and nitrogen sources, respectively. This has been called Facultative CO2 and N2-fixing bacteria in this research. A carbon and nitrogen-free medium has been optimized by carbon-fixing medium and nitrogen-fixing medium in this research (airborne CO2, N2 were taken as the carbon and nitrogen sources, respectively). A strain of CO2 and N2 as the carbon source and nitrogen source, respectively, was isolated by a nitrogen and carbon-free culture medium and has been called HSJ. In addition, the HSJ's characteristics of carbon fixation enzyme activity and nitrogenase activity have been determined. The HSJ with a carbon fixation enzyme(1,5-two ribulose monophosphate carboxylase ) specificity of cbbL gene stripe (cbbL in RubisCO genes) and nitrogen-fixing enzyme specificity of nifH gene stripe was detected by PCR and agarose gel electrophoresis. To testify fixing CO2 and N2 in the air simultaneously, and CO2, N2 as the carbon and nitrogen sources, respectively, validation testing of the bacterium HSJ was carried out. The sequence analysis of 16SrDNA of the HSJ is subordinate to the strains of Streptomyces.

Copyright

© 2016, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Download article (PDF)

TY  - CONF
AU  - Sheng Zhou
AU  - Xiaomin Wang
PY  - 2016/12
DA  - 2016/12
TI  - A Type of Microorganism That Can Fix Greenhouse Gas (CO2 and N2 )Simultaneously-Isolation and Validation Experiment of Facultative CO2 and N2-fixing Bacteria
BT  - Proceedings of the 2016 5th International Conference on Sustainable Energy and Environment Engineering (ICSEEE 2016)
PB  - Atlantis Press
SP  - 422
EP  - 430
SN  - 2352-5401
UR  - https://doi.org/10.2991/icseee-16.2016.78
DO  - 10.2991/icseee-16.2016.78
ID  - Zhou2016/12
ER  -