The Characteristics of Buprofezin Resistant Bacteria From Cassava Rhizosphere

DOI

10.2991/978-94-6463-128-9_37How to use a DOI?

Keywords

Rhizobacteria; Bacillus wiedmanii; Bacillus cereus; ultisol

Abstract

Bacterial isolates from rhizosphere of cassava plant were isolated. The bacterial isolates are resistant to buprofezin. They have the potential to be used as bioremediation agents. The buprofezin-resistant bacteria isolates need to be carried out to determine the morphological, biochemical, Plant Growth Promoting Rhizobacteria (PGPR), molecular, and bioassays characteristics. This study aimed to determine the morphological, biochemical, molecular, PGPR, and bioassay characteristics of KR1 and PA11 isolates. The result showed that KR1 and PA11 were gram-negative and endospore forming bacteria. Biochemical characteristic of KR1 showed positive result for motility and catalase test, glucose fermentation, starch hydrolysis, and MR/VP test, while PA11 showed positive result for glucose, sucrose, and lactose fermentation, starch hydrolysis, MR/VP and oxidase test. KR1 was a fermenter bacteria, while PA11 was an oxidizer/fermenter bacteria based on the O/F test. Molecular characteristics showed KR1 was Bacillus wiedmannii and PA11 was Bacillus cereus. PGPR characteristics showed KR1 was phosphate solubilizing bacteria and PA11 was nitrogen fixing bacteria. Both KR1 and PA11 have ability to produce indole acetic acid (IAA). The bioassay characteristic showed that the consortium application of bacterial isolates (KR1 and PA11 isolates) had a significant effect on the root length of rice plants. The bacterial isolates application showed a better result on plant height than control (without bacterial isolate application).

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Sapto Nugroho Hadi
AU  - Prita Sari Dewi
AU  - Ida Widiyawati
AU  - Yugi R. Ahadiyat
PY  - 2023
DA  - 2023/04/19
TI  - The Characteristics of Buprofezin Resistant Bacteria From Cassava Rhizosphere
BT  - Proceedings of the 3rd International Conference on Sustainable Agriculture for Rural Development (ICSARD 2022)
PB  - Atlantis Press
SP  - 373
EP  - 382
SN  - 2468-5747
UR  - https://doi.org/10.2991/978-94-6463-128-9_37
DO  - 10.2991/978-94-6463-128-9_37
ID  - Hadi2023
ER  -