Genetic Variability Analysis of Terrestrial Spathoglottis plicata Orchid Variants Based on RAPD Marker
- DOI
- 10.2991/assehr.k.210305.011How to use a DOI?
- Keywords
- Spathoglottis plicata, Genetic diversity, PCR-RAPD
- Abstract
The purpose to know the genetic variability of orchid variants Spathoglottis plicata based on molecular marker Random Amplified Polymorphic DNA (RAPD). DNA isolation carried out using TIANGEN Plant Genomic DNA kit. DNA sample was amplified using PCR-RAPD technique. Genetic variability were analyzed using GenAlex 6.1 software to obtain the percentage of polymorphism, genetic distance and principal coordinates. Moreover, NTSYS Version 2.02 Unweighted Pair-Group Method using Arithmetic Average (UPGAMA) was used to create cluster dendrogram based on similarity indeces of S. plicata orchid variants. The result showed that DNA polymorphism of S. plicata orchid variants is 53,28%. Based on the Principal Coordinate Analysis (PCA), orchid variants S. plicata are spread in four quadrants in which the mutant variants on the opposite quadrant with wild type plants. Based on dendrogram, all orchid variants S. plicata are devided into 2 clusters of mutant plants which has a lower similarity index (<0,64) compared to wild type (>70) showing that mutant variants are potential to be new cultivars.
- Copyright
- © 2021, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Fay Della Prika Auvira AU - Ixora Sartika Mercuriani AU - Suyitno Aloysius PY - 2021 DA - 2021/03/08 TI - Genetic Variability Analysis of Terrestrial Spathoglottis plicata Orchid Variants Based on RAPD Marker BT - Proceedings of the 7th International Conference on Research, Implementation, and Education of Mathematics and Sciences (ICRIEMS 2020) PB - Atlantis Press SP - 70 EP - 75 SN - 2352-5398 UR - https://doi.org/10.2991/assehr.k.210305.011 DO - 10.2991/assehr.k.210305.011 ID - Auvira2021 ER -