Identification Human and Animal Blood Mixtures Using Human Cytochrome b Gene

DOI

10.2991/icpsuas-17.2018.81How to use a DOI?

Keywords

Polimerase Chain Reaction (PCR); DNA; human and Animal Blood Mixtures; human cytochrome b

Abstract

Human and animal bloods expecially vertebrata bloods have similar composition so it were difficult to distinguish. The aim of this study was to identify human blood from human and animal blood mixtures using cytochrome b gene that has the species-specific DNA sequence. DNA extracted from variated human and chicken bloods were amplified using human-specific cytochrome b gene primer. The result showed 10 % human blood from mixtures could be identified and DNA extracted from human bloods only could be amplified. The concentration of 0.01 ng still showed DNA band. This primer was very sensitive and specific so it useful for forensic purposes to verify human blood in a blood mixture.

Copyright

© 2018, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Download article (PDF)

TY  - CONF
AU  - Wimbuh Tri Widodo
AU  - Abdul Hadi furqoni
AU  - Ahmad Yudianto
AU  - Sri Puji Astuti Wahyuningsih
PY  - 2017/08
DA  - 2017/08
TI  - Identification Human and Animal Blood Mixtures Using Human Cytochrome b Gene
BT  - Proceedings of the 1st International Conference Postgraduate School Universitas Airlangga : "Implementation of Climate Change Agreement to Meet Sustainable Development Goals" (ICPSUAS 2017)
PB  - Atlantis Press
SP  - 371
EP  - 373
SN  - 2352-5398
UR  - https://doi.org/10.2991/icpsuas-17.2018.81
DO  - 10.2991/icpsuas-17.2018.81
ID  - TriWidodo2017/08
ER  -