Proceedings of the Third International Conference on Separation Technology 2020 (ICoST 2020)

Wound Healing and Antioxidant Activities of Swietenia Macrophylla Seed and Syzygium Aromaticum Bud

Authors
Nur Alaina Baharom, Nur Salsabila Md Norodin, Mohamad Khairul Hafiz Idris, Mohd Amzar Mohamed Zahari, Liza Md Salleh
Corresponding Author
Liza Md Salleh
Available Online 30 December 2020.
DOI
10.2991/aer.k.201229.011How to use a DOI?
Keywords
Swietenia macrophylla, Syzygium aromaticum, antioxidant, cytotoxicity, wound healing
Abstract

This study analysed the wound healing and antioxidant activities of Swietenia macrophylla seed and Syzygium aromaticum bud. The antioxidant activity of Swietenia macrophylla seed and Syzygium aromaticum bud were determined using diphenyl-1-picrylhydrazyl (DPPH) scavenging assay. The cytotoxicity activity using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay against human skin fibroblast 1184 cells was used to determine the relative cell viability of the plant extract meanwhile the wound healing activity was determined by analysing the cell migration using the scratch assay. The highest percentage of antioxidant activity was shown by Syzygium aromaticum bud at 79.28% ± 0.04 compared to 22.50% ± 0.00 by Swietenia macrophylla seed. The highest percentage of relative cell viability for Swietenia macrophylla seed oil was observed at the concentration of 0.01 ppm while Syzygium aromaticum bud at 10 ppm. The highest percentage of wound closure observed from the mixture of 0.1 ppm Swietenia macrophylla seed and 10 ppm Syzygium aromaticum bud at 1:1 volume ratio showed the recovery percentage of 44.60% ± 8.42 at 48 hours after treatment. Meanwhile, the wound closure of Swietenia macrophylla seed and Syzygium aromaticum bud were only 35.46% ± 7.34 and 39.49% ± 6.84 respectively. The finding indicates that the mixture of Swietenia macrophylla seed and Syzygium aromaticum bud able to treat wounds better compared to the sole usage of the plant extracts.

Copyright
© 2020, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the Third International Conference on Separation Technology 2020 (ICoST 2020)
Series
Advances in Engineering Research
Publication Date
30 December 2020
ISBN
978-94-6239-310-3
ISSN
2352-5401
DOI
10.2991/aer.k.201229.011How to use a DOI?
Copyright
© 2020, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

TY  - CONF
AU  - Nur Alaina Baharom
AU  - Nur Salsabila Md Norodin
AU  - Mohamad Khairul Hafiz Idris
AU  - Mohd Amzar Mohamed Zahari
AU  - Liza Md Salleh
PY  - 2020
DA  - 2020/12/30
TI  - Wound Healing and Antioxidant Activities of Swietenia Macrophylla Seed and Syzygium Aromaticum Bud
BT  - Proceedings of the Third International Conference on Separation Technology 2020 (ICoST 2020)
PB  - Atlantis Press
SP  - 75
EP  - 81
SN  - 2352-5401
UR  - https://doi.org/10.2991/aer.k.201229.011
DO  - 10.2991/aer.k.201229.011
ID  - Baharom2020
ER  -