Optimization of Gold Nanoparticles and Molecular Beacon Probe for DNA Detection by Colorimetric Method
- DOI
- 10.2991/ahsr.k.210115.081How to use a DOI?
- Keywords
- Biosensor, Colorimetry, Gold Nanoparticles, Molecular Beacon
- Abstract
Gold nanoparticles are platforms that can be immobilized with a probe; thus, they can be used as biosensors. In the health sector, biosensors are utilized for biomedical applications, such as for DNA marking and cell isolation. In addition, it can be used as a method for Halal Authentication applications for meat products. This study aims to determine the optimum conditions for the synthesis of gold nanoparticles as a platform for immobilization and application of AuNPs-MB as biosensors with the colorimetry method for Halal Authentication applications of meat product. This research optimized gold nanoparticles using 6 series of HAuCl4 concentration, namely 0.0635; 0.127; 0.19; 0.254; 0.635; dan 1.27 (mM). The most optimum concentration was immobilized with probe MB and measured using Uv-Vis Spectrophotometer to identify the absorption and Scanning Electron Microscope (SEM) to identify nanoparticles’ size distribution. The results showed that 0.19 mM of AuNPs gave the best results, which was stable for 3.5 months and had good results measured using SEM with an average size of 21.7 nm. The value of absorbance, before immobilization, decreased from 0.226 to 0.167 and indicated that the prose was successful. Based on the result of this study, it can be concluded that AuNPs-MB was proven to be a biosensor for Halal Authentication applications using the colorimetric method.
- Copyright
- © 2021, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Hari Widada AU - Mar’atus Sholikhah AU - Sabtanti Harimurti AU - Dyani Primasari Sukamdi AU - Abdul Rohman AU - Riris Istighfari Jenie AU - Sismindari PY - 2021 DA - 2021/01/16 TI - Optimization of Gold Nanoparticles and Molecular Beacon Probe for DNA Detection by Colorimetric Method BT - Proceedings of the 4th International Conference on Sustainable Innovation 2020–Health Science and Nursing (ICoSIHSN 2020) PB - Atlantis Press SP - 408 EP - 411 SN - 2468-5739 UR - https://doi.org/10.2991/ahsr.k.210115.081 DO - 10.2991/ahsr.k.210115.081 ID - Widada2021 ER -