Correlation Between Ratio of Purine Derivative: Creatinine Concentrations in Spot Urine Sampling with Total Urinary Excretion of Purine Derivative in Garut Rams and Ewes

DOI

10.2991/absr.k.220305.014How to use a DOI?

Keywords

Purine Derivative Excretion; Spot Sampling; Rumen Microbial Protein Synthesis; Garut Sheep

Abstract

This study aimed to determine the correlation between ratio of purine derivative:creatinine concentrations by spot sampling method with total urinary excretion of purine derivative (PD) in Garut rams and ewes, as well as knowing the right sampling time to predict microbial protein synthesis in Garut rams and ewes. There were six Garut rams and six Garut ewes placed in metabolic cages and fed with elephant grass and bran pollard with a ratio of 60:40 (based on the dry matter). This research was conducted 14 days for the adaptation period and five days for the collection period. The urine samples in the collection period used the spot sampling method by taking urine periodically at intervals of three hours a day (24 hours). Urine samples obtained were analyzed for creatinine and purine derivatives content (allantoin, uric acid, and xanthin-hypoxanthine). The data was analyzed using an Independent Student t-test design between Garut rams and ewes. The ratio of purine derivatives (PD): creatinine (PDC index) was correlated with the total daily excretion of purine derivatives (PD), hence the best time for spot sampling could be implemented. Based on the research, concentration of urinary PD and creatinine in Garut rams were higher than ewes. Excretion of allantoin, xanthinhypoxanthine and creatinine in Garut rams were also higher than ewes. The concentration nor excretion of urinary PD in rams and ewes before being divided by metabolic body weight showed significant differences. The best time for spot sampling applied to Garut rams was six to nine hours after afternoon feeding with the equation Y=6.802X-26.04, while for Garut ewes was six to nine hours after afternoon feeding with the equation Y=3,630X+43,79. In conclusion, the concentration and total excretion of urinary PD in Garut rams were higher than the ewes. There were differences in the regression equation formula, to predict PD excretion based on PD concentration corrected by creatinin content of urine spot sampling with the similar best sampling time at the range of six to nine hours after afternoon feeding.

Copyright

© 2022 The Authors. Published by Atlantis Press International B.V.

Open Access

This is an open access article under the CC BY-NC license.

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TY  - CONF
AU  - Titi Widya Ningrum
AU  - Chusnul Hanim
AU  - Lies Mira Yusiati
PY  - 2022
DA  - 2022/03/10
TI  - Correlation Between Ratio of Purine Derivative: Creatinine Concentrations in Spot Urine Sampling with Total Urinary Excretion of Purine Derivative in Garut Rams and Ewes
BT  - Proceedings of the 2nd International Conference on Smart and Innovative Agriculture (ICoSIA 2021)
PB  - Atlantis Press
SP  - 93
EP  - 99
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.220305.014
DO  - 10.2991/absr.k.220305.014
ID  - Ningrum2022
ER  -