Antibacterial Activity of Ketapang (Terminalia cattapa L.) Leaf Extract Against Staphylococcus aureus and Pseudomonas aeruginosa Isolates of Diabetic Wounds

DOI

10.2991/978-94-6463-018-3_13How to use a DOI?

Keywords

Terminalia cattapa L; antibacterial activity; Staphylococcus aureus; Pseudomonas aeruginosa; MRSA

Abstract

This research aims at measuring antibacterial activity differences of ethanolic extract of ketapang leaf in the concentrations of 10 mg/mL, 25 mg/mL, 50 mg/mL, 75 mg/mL, and 100 mg/mL against S. aureus ATCC 6538, Staphylococcus aureus Methycilin Resistance (MRSA), and P. aeruginosa isolates of diabetic wounds. The ethanolic extraction of ketapang leaf was made using a maceration method. Antibacterial activity test was conducted based on the use of agar well diffusion assay method with Mueller Hinton Agar media, while the Minimum Inhibitory Concentration (MIC) and Minimum bactericidal concentration (MBC) were conducted using Mueller Hinton Broth media via microdilution method. The antibacterial activity was calculated based on MIC and MBC. The ketapang leaf dry extract with ethanol solvent resulted in paste as much as 13.6%. The higher the concentration of ketapang leaf extract, the higher its inhibiting power to those three bacterial types (S. aureus ATCC 6538, MRSA and P. aeruginosa). The highest inhibiting power of ketapang leaf extract was to MRSA (21.6 ± 0.8944) mm, while the smallest was to P. aeruginosa (16.0 ± 0.7071) mm. The MIC and MBC values of P. aeruginosa were 6.25 mg/mL, while those of S.aureus ATCC 6538 and MRSA were 50 mg/mL. The results of this research can provide information related to the ketapang leaf extract with ethanol solvent, while both Gram-positive and Gram-negative bacteria can be used as the antibacteria.

Copyright

© 2023 The Author(s)

Open Access

Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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TY  - CONF
AU  - Sri Darmawati
AU  - Lutfi Kurniasih
AU  - Hira Nur Aulya Safitri
AU  - Bayu Setyo Pratomo
AU  - Muhammad Evy Prastiyanto
PY  - 2022
DA  - 2022/12/15
TI  - Antibacterial Activity of Ketapang (Terminalia cattapa L.) Leaf Extract Against Staphylococcus aureus and Pseudomonas aeruginosa Isolates of Diabetic Wounds
BT  - Proceedings of the First International Conference on Medical Technology (ICoMTech 2021)
PB  - Atlantis Press
SP  - 93
EP  - 101
SN  - 2468-5739
UR  - https://doi.org/10.2991/978-94-6463-018-3_13
DO  - 10.2991/978-94-6463-018-3_13
ID  - Darmawati2022
ER  -