Isolation and Activity of Amylase Enzyme in Isolates of Fungi From Black Rice Lemang (Oryza sativa Siarang)

DOI

10.2991/absr.k.200807.011How to use a DOI?

Keywords

Black rice, Isolation, Identification, Descriptive Survey, Amylolytic Mushroom, Amylolytic Index

Abstract

Black rice Lemang (Oryza sativa Siarang) from Kenagarian Talang Babungo is one of the Minangkabau specialties. Lemang left moldy to get a different taste from ordinary lemang. This fungus occurs in chemical processes that are assisted by the amylase enzyme that functions to break down starch into simple sugar units. The purpose of this research was to find the type of fungus found in black rice lemang and to find out the activity of the fungus amylase enzyme which was isolated from black rice lemang. The study was conducted at the Microbiology Laboratory of Andalas University, Padang from March to April 2016. This study used a descriptive survey method by isolating fungi, making pure seedlings, and then identifying and descriptive methods by growing fungi isolates on rice flour medium (ATB) to identify the amylase fungal enzymes that were characterized. by the formation of open areas around the fungal colonies. The results of isolation and identification found five varieties of mold in lemang, namely Aspergillus niger, Aspergillus ustus, Aspergillus sp, Mucor racemosus, and Penicillium expansum. The highest amylolytic index is Penicillium expansum (1.5) and the lowest amylolytic index is Mucor racemosus (0.5).

Copyright

© 2020, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Mades Fifendy
AU  - Periadnadi
AU  - Gustina Indriati
AU  - Syovia Osnita
AU  - Lara Annisa
PY  - 2020
DA  - 2020/08/07
TI  - Isolation and Activity of Amylase Enzyme in Isolates of Fungi From Black Rice Lemang (Oryza sativa Siarang)
BT  - Proceedings of the International Conference on Biology, Sciences and Education (ICoBioSE 2019)
PB  - Atlantis Press
SP  - 46
EP  - 50
SN  - 2468-5747
UR  - https://doi.org/10.2991/absr.k.200807.011
DO  - 10.2991/absr.k.200807.011
ID  - Fifendy2020
ER  -