Proceedings of the 2017 5th International Conference on Mechatronics, Materials, Chemistry and Computer Engineering (ICMMCCE 2017)

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Study on Extraction Technology and Functional Activity of Sika Deer Velvet(Residue) Collagen

Authors
Shiru Bo, Xiaolin Zhang, Quankai Wang
Corresponding Author
Shiru Bo
Available Online September 2017.
DOI
10.2991/icmmcce-17.2017.20How to use a DOI?
Keywords
Sika Deer Velvet Antler; Collagen; Functional Activity
Abstract

Objective:Extraction method and functional activity of collagen that is extracted from residue that is insoluble in any medium polar solvent by ultrasonic extraction andsupercritical CO2 extraction are studied. Finally the optimal enzymolysis conditionsare obtained.Methods: Quantitative content of collagen in deer antler (residue) by quantitate hydroxyproline. Sika deer antler collagen is prepared by enzymolysis of papain. According to single factor test and the orthogonal design, the optimal enzymatic conditions aredetermined by the extraction rate of collagen as evaluation indicator. And functional activity are tested which include antioxidant capacity, hydroxyl free radical scavenging ability, DPPH scavenging ability of the test and keratinocyte cell test.Results: The content of collagen in the deer antler (residue) is 4.5795%,the best extraction process is 2% optimum enzyme dosage , PH 6.0, 1:20 solid-liquid ratio, enzymolysis time 66h at 10 . The extraction rate of collagen of the deer antler is 54.59%.The protein is collagen by UV spectroscopy.Functional tests are showed a good functionality and growth and reproduction capacity.

Copyright
© 2017, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Volume Title
Proceedings of the 2017 5th International Conference on Mechatronics, Materials, Chemistry and Computer Engineering (ICMMCCE 2017)
Series
Advances in Engineering Research
Publication Date
September 2017
ISBN
978-94-6252-381-4
ISSN
2352-5401
DOI
10.2991/icmmcce-17.2017.20How to use a DOI?
Copyright
© 2017, the Authors. Published by Atlantis Press.
Open Access
This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

Cite this article

risenwbib
TY  - CONF
AU  - Shiru Bo
AU  - Xiaolin Zhang
AU  - Quankai Wang
PY  - 2017/09
DA  - 2017/09
TI  - Study on Extraction Technology and Functional Activity of Sika Deer Velvet(Residue) Collagen
BT  - Proceedings of the 2017 5th International Conference on Mechatronics, Materials, Chemistry and Computer Engineering (ICMMCCE 2017)
PB  - Atlantis Press
SP  - 94
EP  - 100
SN  - 2352-5401
UR  - https://doi.org/10.2991/icmmcce-17.2017.20
DO  - 10.2991/icmmcce-17.2017.20
ID  - Bo2017/09
ER  -