Research on the Impact of Transplantation of Oligodendrocyte Precursor Cells on the Recovery of Spinal Cord Injury in a Rat Model
- DOI
- 10.2991/icmcm-16.2016.105How to use a DOI?
- Keywords
- Spinal Cord Injury; Oligodendrocyte Precursor Cells; Rat Model
- Abstract
Objective: To investigate the impact of transplantation of oligodendrocyte precursor cells on the recovery of spinal cord injury in a rat model. Methods: The cerebral cortices of 48-hour neonate rats were harvested for the OPCs primary culture. The growth of OPCs in vitro was observed consecutively under the contrast phase microscope and scanning electron microscope. After the primary culture in vitro, the OPCs were further dissociated by the shaking process and differential adhesion. Seven days after spinal cord injury , the rats of transplantation group received OPCs transplantation, of control group were injected with equivalent saline , and of injury -only group were untreated. The effects of OPCs transplantation on neural functional recovery in spinal cord injured rats and matosensory evoked potentials (SEP) were assessed. Results: the simple morphology of OPCs progressively evolved to more complex forms with profuse outgrowth of elongated processes and extensive secondary branching. The performance of MEP and SEP the normal injured rats were worse than the sham group. Conclusion: The transplantation of OPCs may be helpful to the recovery of neural function of rats.
- Copyright
- © 2016, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Meng Zhang AU - Hongbo Yao PY - 2016/12 DA - 2016/12 TI - Research on the Impact of Transplantation of Oligodendrocyte Precursor Cells on the Recovery of Spinal Cord Injury in a Rat Model BT - Proceedings of the 2016 7th International Conference on Mechatronics, Control and Materials (ICMCM 2016) PB - Atlantis Press SP - 546 EP - 550 SN - 2352-5401 UR - https://doi.org/10.2991/icmcm-16.2016.105 DO - 10.2991/icmcm-16.2016.105 ID - Zhang2016/12 ER -