Biodegradation of Spilled-oil by Immobilization Microorganisms on Diatomite-based Carrier Material

DOI

10.2991/icimm-15.2015.282How to use a DOI?

Keywords

biodegradation; oil spill; adsorption; immobilization; microorganism

Abstract

This paper focused on research the dynamic adsorption capacity of immobilized microorganisms on diatomite-based carrier material. Several important parameters were studied, including different immobilization methods, pH value of cross linking agent, the influence on the physical properties and degradation effect of immobilized microspheres with various immobilization bacteria amount. The experimental results showed that direct immobilization method was better that of the other methods. After crosslinking at the temperature of 25 C for 24 h, the best immobilization conditions were at pH 5-6 in the cross-linking agent solution. The volume ratio of immobilization bacteria liquid and gel solution was 1:5. The immobilization of inorganic salts could accelerate the recovery of the activity of immobilized microorganisms, and the surface structure of the microspheres was observed before and after degradation by SEM. The highest degradation rate could reach 40% for free M4, while 70% for immobilized M4, which increased oil degradation rate over 30%.

Copyright

© 2015, the Authors. Published by Atlantis Press.

Open Access

This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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TY  - CONF
AU  - Xiaoyue Wang
AU  - Shaojun Zhang
AU  - Chunjuan Wang
PY  - 2015/07
DA  - 2015/07
TI  - Biodegradation of Spilled-oil by Immobilization Microorganisms on Diatomite-based Carrier Material
BT  - Proceedings of the 5th International Conference on Information Engineering for Mechanics and Materials
PB  - Atlantis Press
SP  - 1541
EP  - 1546
SN  - 2352-5401
UR  - https://doi.org/10.2991/icimm-15.2015.282
DO  - 10.2991/icimm-15.2015.282
ID  - Wang2015/07
ER  -