Study on Accurate Determination of Volatile Fatty Acids in Rumen Fluid by Capillary Gas Chromatography
Authors
Cheng Luo, Suyi Cai, Linyan Jia, Xiang Tang, Ruinan Zhang, Gang Jia, Hua Li, Jiayong Tang, Guangmang Liu, Caimei Wu
Corresponding Author
Cheng Luo
Available Online July 2015.
- DOI
- 10.2991/icimm-15.2015.73How to use a DOI?
- Keywords
- Capillary gas chromatography; Rumen fluid; Volatile fatty acids; Internal standard method
- Abstract
In order to obtain accurate results of volatile fatty acids (VFAs) in rumen fluid by capillary gas chromatography (GC), a pretreatment method was firstly conducted by dissolving VFAs in the methanol. An internal standard method (ISM) with crotonic acid as the internal standard was adopted in the detection. The results showed that the reproducibility of VFA (acetic acid, propanoic acid and butyric acid) was enhanced remarkably with methanol added as the solvent. The reproducibility was dependent on both of the VFA concentration and methanol amount. In conclusion, methanol solvent was more suitable than aqueous solvent for VFA accurate detection with ISM by capillary GC.
- Copyright
- © 2015, the Authors. Published by Atlantis Press.
- Open Access
- This is an open access article distributed under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
Cite this article
TY - CONF AU - Cheng Luo AU - Suyi Cai AU - Linyan Jia AU - Xiang Tang AU - Ruinan Zhang AU - Gang Jia AU - Hua Li AU - Jiayong Tang AU - Guangmang Liu AU - Caimei Wu PY - 2015/07 DA - 2015/07 TI - Study on Accurate Determination of Volatile Fatty Acids in Rumen Fluid by Capillary Gas Chromatography BT - Proceedings of the 5th International Conference on Information Engineering for Mechanics and Materials PB - Atlantis Press SP - 386 EP - 391 SN - 2352-5401 UR - https://doi.org/10.2991/icimm-15.2015.73 DO - 10.2991/icimm-15.2015.73 ID - Luo2015/07 ER -