Proceedings of the 1st International Conference for Health Research – BRIN (ICHR 2022)

Effect of Sterilization on Protein Adsorption of Micro- and Nano-sized Aluminum Hydroxide Adjuvant

Authors
Damai Ria Setyawati1, *, Sjaikhurrizal El Muttaqien1, Donny Ramadhan2, Etik Mardliyati1
1Research Center for Vaccine and Drugs Development, National Research and Innovation Agency (BRIN), Jakarta, Indonesia
2Research Center for Pharmaceutical Ingredients and Traditional Medicine, National Research and Innovation Agency (BRIN), Jakarta, Indonesia
*Corresponding author. Email: dama004@brin.go.id
Corresponding Author
Damai Ria Setyawati
Available Online 1 March 2023.
DOI
10.2991/978-94-6463-112-8_75How to use a DOI?
Keywords
adjuvant; aluminum hydroxide; adsorption; sterilization
Abstract

Aluminum hydroxide (AH) is one of the aluminum-based adjuvants that forms needle-like crystalline structure with the average particle size of 1–20 µm. Due to their favorable safety profile, AH adjuvant has been used widely in various human vaccine in diphtheria, tetanus, pertussis, hepatitis, and papilloma virus vaccines. The antigen-adjuvant interaction is strongly governed by the adjuvant’s physicochemical characters, such as shape, dimension and surface charge. Based on this concept, tremendous efforts to amplify the performance of the adjuvant have been made by modifying its particle size into nano-sized adjuvant. Also, during vaccine formulation, several physical processes may take place and potentially affect the antigen-adjuvant interaction, such as dilution, salt and aging exposure, pH change, heat, pressure and shear forces stress. Therefore, in this study we have examined the effect of sterilization and pH of AH nanoparticle (AHNp) with the average size of ~160 nm on its physical characterization and adjuvant-antigen interaction. The obtained results were compared with those from AH microparticle (AHMp) and commercial AH adjuvant (AHC).

AHMp and AHNp were prepared by using common precipitation method through the addition of alkali to the solution of aluminum chloride. The aluminum content of both AH suspension is 12.1 mg/mL. The average particle size of AHMp and AHNp by static light scattering are 4.21 µm and 0.16 µm, respectively. For evaluating the effect of autoclave on AH properties, all AH samples on glass container were autoclaved at 121 ℃ for 30 dan 60 min. The result shows that all samples did not give significant physical character change after autoclave. All measured parameters including the solid content of AH suspension, the acquired mass after freezing dry, pH value, and visual appearance remained the same as those before autoclave. The X-ray diffractogram of AHMp and AHNp revealed that the diffraction bands were sharper after autoclaving.

Following autoclaving, all fraction of (bovine serum albumin) BSA added into the adjuvant could be completely adsorbed by AHNp, AHMp, and AHC samples, as high as its pre-autoclaved condition, achieving maximum adsorption capacity. Such phenomena could be obtained even when volume ratio of adjuvant/BSA multiplied 20-times. It indicates that the binding efficiency BSA to all adjuvant samples was not affected by the autoclaving in all given test condition. On the other hand, the pH of AH suspension significantly affects the protein adsorption capacity. The highest binding efficiency of AH-protein could be achieved in physiological pH condition, highlighting the pH-dependency of AH-protein interaction.

Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

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Volume Title
Proceedings of the 1st International Conference for Health Research – BRIN (ICHR 2022)
Series
Advances in Health Sciences Research
Publication Date
1 March 2023
ISBN
978-94-6463-112-8
ISSN
2468-5739
DOI
10.2991/978-94-6463-112-8_75How to use a DOI?
Copyright
© 2023 The Author(s)
Open Access
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

Cite this article

TY  - CONF
AU  - Damai Ria Setyawati
AU  - Sjaikhurrizal El Muttaqien
AU  - Donny Ramadhan
AU  - Etik Mardliyati
PY  - 2023
DA  - 2023/03/01
TI  - Effect of Sterilization on Protein Adsorption of Micro- and Nano-sized Aluminum Hydroxide Adjuvant
BT  - Proceedings of the 1st International Conference for Health Research – BRIN (ICHR 2022)
PB  - Atlantis Press
SP  - 824
EP  - 832
SN  - 2468-5739
UR  - https://doi.org/10.2991/978-94-6463-112-8_75
DO  - 10.2991/978-94-6463-112-8_75
ID  - Setyawati2023
ER  -